摘要
目的建立高效液相色谱(HPLC)法测定血浆异丙酚浓度,研究异丙酚在不同年龄组全麻手术患者体内的药代动力学。方法将20例ASA评级为Ⅰ～Ⅱ级的择期手术患者按年龄分成两组,青壮年组10例,平均年龄(40±9)岁,老年组10例,平均年龄(68±7)岁,均静脉注射异丙酚2mg/kg后不同时间取血,以HPLC法测定血浆异丙酚浓度,应用3P87软件包计算药动学参数,SPSS软件包进行统计分析。结果20例患者异丙酚的药动学特征均符合三室开放模型,老年组与青壮年组比较,Vc明显减少(P<0.05),Cl显著下降(P<0.05),k31明显变小(P<0.05),t1/2β也较长(P<0.05)。结论HPLC法测定血浆异丙酚浓度简便、准确,灵敏度高,重现性好,适用于临床异丙酚血药浓度监测及药动学研究。与青壮年比较,老年人由于中央分布容积减少和清除率下降,同等剂量应用时血药浓度明显偏高,提示异丙酚在老年人麻醉用药时需减量。
Objective To establish a HPLC method for determining propofol concentration in plasma, and to study the pharmacokinetics of propofol in the patients of different age groups under general anesthesia. Methods 20 ASA Ⅰ～Ⅱ patients undergoing selective operation were divided into 2 groups, the young group （10 cases） and the old group （10 cases）. After intravenous administration of propofol 2 mg/ kg, the venous blood samples were taken at various time points to determine the plasma concentrations of propofol by HPLC. The data were analyzed with 3P87 program to get pharmacokinetic parameters. Results The pharmacokinetics of propofol in the 20 patients were best described by a three compartment pharmacokinetic model. Vc and Cl were smaller and t1/2β was longer in the old group as compared with the young group. Conclusion HPLC is rapid, simple, accurate and sensitive to determine propofol concentration in plasma. It is suitable for monitoring propofol and pharmacokinetic study. Since Vc and Cl decrease in the elderly, the plasma concentration of propofol is higher, which indicates that propofol should be reduced in dose for the old patients.
出处
《中国药业》
CAS
2010年第3期11-12,共2页
China Pharmaceuticals
