摘要
目的:比较新疆紫草根及茎残基部分成分及含量。方法:采用薄层色谱法及HPLC指纹图谱鉴别新疆紫草成分;紫外-可见分光光度法测定新疆紫草中羟基萘醌总色素含量;HPLC测定β-β'-二甲基丙烯酰阿卡宁和紫草素的含量总和。结果:10批药材根与茎残基部分薄层斑点数目、形状一致,根的斑点大、色泽深;指纹图谱中两者指纹特征相同,相同保留时间下,根的峰面积更大;10批药材中根的羟基萘醌总色素含量平均为3.631%,茎残基平均为1.516%,紫草素与β-β'-二甲基丙烯酰阿卡宁的含量总和的平均值在根及茎残基中分别为0.896%、0.309%。结论:新疆紫草根与茎残基部分所含成分基本一致,但根中羟基萘醌总色素含量、β-β'-二甲基丙烯酰阿卡宁与紫草素总含量均高于茎残基部分两倍以上。
Objective： To compare the composition and content of Arnebiae Radix and the stem residues.Methods： TLC and HPLC were used to identify Arnebia,ultraviolet-visible spectrophotometry was used to determine the content of hydroxy naphthoquinone total pigment in Arnebia,HPLC was used to determine the total content of β-β′-dimethyl acrylamide Aka Ning and shikonin.Results： The number of spots of Arnebia Radix was consistent with that of the stem residues in 10 batches of medicinal materials,the former was larger and deeper in color.Their features of fingerprint are the same,at the same retention time,the peak area of radix was larger;The average content of hydroxy naphthoquinone total pigment was 3.631% in the radix,and 1.516% in the stem.The total content of β-β′-dimethyl acrylamide Aka Ning and shikonin in the radix and the stem were respectively 0.89% and 0.309%.Conclusion： The ingredients in the radix and the stem residues are the same,but the contents of root of the total pigment content of hydroxyl-naphthoquinone,β-β′-dimethyl acrylamide Aka Ning and shikonin are twice higher than those of the stem residues.
出处
《中药材》
CAS
CSCD
北大核心
2012年第3期361-366,共6页
Journal of Chinese Medicinal Materials
基金
国家"重大新药创制"科技重大专项(2009ZX09103-430)
