摘要
目的 研究宫颈癌组织中癌细胞中的共刺激分子B7 1、B7 2、细胞间粘附因子 Ⅰ (ICAM 1)及主要组织相容性抗原 (MHC)Ⅰ、Ⅱ类分子表达 ,以揭示人体肿瘤的逃逸机制。方法 利用免疫组织化学技术 ,观察了42例人宫颈癌组织中B7 1、B7 2、ICAM 1的表达 ,观察了 36例MHCⅠ、Ⅱ类抗原的表达 ;利用原位杂交技术观察了 2 0例人宫颈癌组织中B7 1、B7 2和ICAM 1的mRNA表达。结果 在进行免疫组织化学检测的标本中 ,42例中有 2 2及 2 7例的癌组织癌细胞分别表达B7 1及ICAM 1分子 ,但无一例表达B7 2分子 ;所有癌组织及癌巢间质中均可见有程度不同的B7 1、B7 2和ICAM1阳性的树突状细胞及淋巴细胞浸润 ;37例中有 32例及 4例的癌细胞分别表达MHCⅠ和Ⅱ类抗原 ,癌巢及间质内有成团或散在的MHCⅡ阳性树突状细胞浸润 ,同一标本中MHCⅡ阳性树突状细胞的数量明显多于B7 1和B7 2阳性树突状细胞。在进行原位杂交的 2 0例标本中 ,有 11例和 15例的癌细胞分别表达B7 1和ICAM 1mRNA ,浸润的树突状细胞及淋巴细胞可见B7 1、B7 2和ICAM 1mRNA表达。结论 人宫颈癌的癌细胞确实表达与抗原提呈及刺激T细胞活化有关的分子 ,而且肿瘤组织中存在有职业性抗原提呈细胞浸润 。
Objective To explore the immune escape mechanism by investigating the expression of B7 1, B7 2, ICAM 1 and MHCⅠ, MHCⅡ Ag in the cancer cells of cervical carcinoma Methods The expressions of B7 1, B7 2 and ICAM 1, together with MHCⅠ and ⅡAg were analyzed in tumor cells and interstitial cells of human cervical carcinoma with immunohistochemistry technique The mRNA expressions of B7 1,B7 2 and ICAM 1 were observed by in situ hybridization Results Out of the 42 fresh specimens analyzed with immunohistochemistry, the tumor cells expressed B7 1 Ag and ICAM 1 molecules in 22 and 27 specimens respectively, but not expressed B7 2; B7 1 +, B7 2 + and ICAM 1 + dendritic cells and lymphocytes were scattered more or less in the parenchyma and interstitum Among the 37 cases studied for MHC Ag expression, the tumor cells expressed MHCⅠ Ag and MHCⅡAg in 32 and 4 cases respectively No matter whether the tumor cells expressed MHCⅡAg or not, MHCⅡAg + dendritic cells were observed in the nests and the interstitial When the same section staind for identifying, B7 1, B7 2 and MHCⅡAg expressions was compared, by and large, the MHCⅡAg + dendritic cells were always more numerous than B7 1,B7 1 + or B7 2 + ones As for the 20 specimens inspected with in situ hybridization, the tumor cells in 11 and 12 cases expressed B7 1 mRNA and ICAM 1 mRNA respectively; Infiltrating dendritic cells and lymphocytes expressed B7 1 mRNA, B7 2 mRNA and ICAM 1 mRNA Conclusion Tumor cells in most cervical carcinomas have the necessary elements for presenting antigen and evoking T lymphocytes activation, and there are a lot of dendritic cells infiltrated in the tumor tissues These findings indicate that the mechanism for tumor escape may be attributed to the post antigen presentation events
出处
《中华医学杂志》
CAS
CSCD
北大核心
2000年第5期342-345,共4页
National Medical Journal of China
基金
国家自然科学基金资助项目 !(39470 2 93)
