摘要
目的:观察不同转移潜能人乳腺癌细胞株中Twist及其相关调控基因mRNA表达的差异。方法:培养低侵袭性人乳腺癌细胞株MCF-7和高转移性人乳腺癌细胞株MDA-MB-435S,采用RT-PCR法检测两种细胞株中Twist、脆性组氨酸三联体(FHIT)、皮性钙黏附素(E-cadherin)、细胞凋亡相关基因Mcl-1、Bcl-2和Bax mRNA表达的差异。结果:MCF-7中FHIT、E-cadherin、Bax mRNA的表达显著高于MDA-MB-435S细胞,Twist mRNA在MDA-MB-435S细胞中的表达明显高于MCF-7,Mcl-1、Bcl-2 mRNA在两种细胞株中的表达无明显差异。结论:Twist、FHIT、E-cadherin、Bax mRNA表达差异可能与人乳腺癌细胞的不同转移潜能有关。
Objective ： To observe the differences of expressions of Twist and its related regulatory genes mRNA in human breast cancer cells with different metastatic potentials. Methods： Minimal - invasive human breast cancer MCF - 7 cell line and high metastatic human breast cancer MDA -MB -435S cell line were cultured, RT -PCR was used to detect the expressions of Twist, fragile histidine triad （FHIT） , E - cadherin, Mcl - 1, Bc1-2, and Bax mRNA in MCF -7 cell line and MDA - MB -435S cell line. Results： The expression levels of FHIT, E - cadherin, and Bax mRNA in MCF -7 cell line were significantly higher than those in MDA - MB -435S cell line. The expression of Twist mRNA in MDA- MB -435S cell line was significantly higher than that in MCF- 7 cell line; there was no significant difference in the expression levels of Mcl- 1 and Bcl- 2 mRNA between MCF- 7 cell line and MDA -MB -435S cell line. Conclusion： The differences of expression levels of Twist, FHIT, E - cadherin, and Bax mRNA may be related to different metastatic potentials of human breast cancer cells.
出处
《中国妇幼保健》
CAS
北大核心
2012年第17期2664-2666,共3页
Maternal and Child Health Care of China
基金
四川省科技厅科研项目〔No.2009HH0014〕
