摘要
目的研究不同剂型亚甲基蓝(methylene blue,MB)介导的光动力疗法(photodynamic therapy,PDT)对体外培养的人牙周膜成纤维细胞(human periodontal ligament fibroblasts,HPDLFs)的毒性作用。方法将体外培养的HPDLFs分别加入两种不同剂型MB中(水剂型和混合剂型,均设置5组浓度,分别为0.1、1.0、10、25、50μmol/L),孵育10min,波长670nm半导体激光照射5min,输出功率40mW,功率密度10mW/cm2。光照结束后继续培养24h,用噻唑蓝(MTT)比色法检测细胞毒性,光镜下观察细胞形态学变化。结果两种不同剂型MB介导的PDT对体外培养的HPDLFs的毒性作用未见明显差异(P>0.05)。当MB浓度≤25μmol/L时,细胞毒性为1～2级,光镜下观察细胞未发现明显形态学改变;浓度>25μmol/L时,细胞毒性为3级,并可观察到细胞皱缩、变圆。结论 PDT对HPDLFs的毒性作用受光敏剂浓度影响,适当浓度的两种剂型MB介导的PDT对体外培养的HPDLFs影响轻微,且两种剂型MB介导的PDT对体外细胞毒性无差异。
Objective To investigate the photodynamic effect of two different formulations of methylene blue （MB） on the killing ability to human periodontal ligament fibroblasts（HPDLFs）in vitro. Methods Cultured HPDLFs were incu- bated in a medium containing two different formulations of MB with 5 different concentration（0.1,1.0, 10,25,50μmol/ L） for 10 minutes, and irradiated 5 minutes using a semiconductor laser（wavelength 670 nm, output power 40 mW, ener- gy density l0 mW/cm2）. After treatment, the cells continued to be cultivated for 24 hours, and then the cytotoxicity was investigated by light microscope and MTT assay. Results There was no significant difference in the cytotoxicity of the two different formulations of MB （P 〉 0.05）. When the concentration of the photosensitizers was no more than 25 μmol/L, the ceils were normal in appearance and cytotoxicity score was at 1-2 grade; when the concentration was more than 25 ttool/L, the cells shrank and became round, and cytotoxicity score was grade 3. Conclusion There is no significant difference in the cytotoxicity of the two different formulations of MB. The cytotoxicity is affected by the concentration of the photosensitizers. When using appropriate drug concentration, the cell has a lower toxicity.
出处
《中国实用口腔科杂志》
CAS
2012年第4期214-218,共5页
Chinese Journal of Practical Stomatology
基金
福建省自然科学基金(2010J01159)
关键词
亚甲基蓝
光动力疗法
人牙周膜成纤维细胞
细胞毒性
methylene bue
photodynamic therapy
human periodontal ligament fibroblasts
cytotoxicity
