摘要
目的探讨急性早幼粒细胞白血病患者化疗前后外周血中单个核细胞膜表面TLR2、TLR4对NK细胞和T细胞上CD95L表达的影响。方法运用流式细胞术,对NK细胞和CD14+细胞进行设门,检测急性早幼粒细胞白血病患者和健康对照组单个核细胞膜表面TLR2+、TLR4+、CD3+CD95L+、NK+CD95L+,进行比较分析。结果与对照组比较,治疗前组CD3+细胞明显增加,NK+CD95L+、CD3+CD95L+细胞数下降,统计学上有显著性差异(P<0.05);治疗后组CD14+、TLR2+、TLR4+、NK+、CD3+CD95L+细胞明显减少,有显著性差异(P<0.05)。与治疗前组相比,治疗后组CD14+、TLR2+、TLR4+、CD3+细胞明显减少,NK+CD95L+细胞明显增加,有显著性差异(P<0.05)。结论 APL患者外周血中单核细胞上TLR2、TLR4的表达影响NK细胞的CD95L诱导凋亡的能力,在抗肿瘤逃避机体免疫中是一种有益尝试。
Objective To explore the effect of TLR2 and TLR4 on CD95L level on INK cells and T cells surface in acute promyelocytic leukemia patients before and after treatment. Methods The TLR2 ＋ ,TLR4 ,CD3 CD95L and NK ＋ CD95L level on mononuelear cell surface were detected and analysed in acute promyelocytic leukemia patients group and healthy control group by flow cytometry. Results The ratio of CD3 cells was increased and the ratio of CD3 CD95L ＋ and NK CD95L ＋ ceils were decreased in treatment group significantly compared to healthy group（P 〈0.05）. The ratio of CD14 ,TLR2 ＋ ,TLR4 ＋ ,NK ＋ ,CD3 CD95L cells were decreased significantly in after treatment group compared to healthy group （ P 〈 0.05 ）. The ratio of CD14 ＋ , TLR2 ＋ , TLR4 ＋ , CD3 cells were decreased and the ratio of NK CD95L＋ cells was increased separately in treatment group compared to before treatment group（ P 〈 0.05 ）. Conclusion The expression of TLR2 and TLR4 affected inducing apoptosis capability of CD95L on NK cells in acute promyelocytic leukemia patients. It is a good assay on tumor immune escape research.
出处
《医学研究杂志》
2012年第5期102-105,共4页
Journal of Medical Research
基金
绍兴市科技资助项目(2009A33030)
