摘要
目的探讨消黄方对二甲基亚硝胺(dimethylnitrosamine,DMN)肝纤维化大鼠肝组织氧化应激的影响。方法雄性Wistar大鼠30只,随机分为正常组及DMN模型组,DMN模型组大鼠DMN(10 mg/kg)每周前3天连续腹腔注射共4周制备大鼠肝纤维化模型,造模第3周,DMN大鼠随机分为模型对照组和消黄方组(例数10),消黄方组每天造模的同时给予方剂煎出液灌胃2周。造模4周末,处死全部大鼠,获取肝组织,检测肝组织羟脯氨酸(hydroxy prone,Hyp)含量,检测肝组织超氧化物歧化酶活性(superoxidedismutase,SOD)、谷胱甘肽转移酶(glutathione-S-transfcrase,GST)活性、谷胱甘肽(glutathione,GSH)及丙二醛含量(malondialdehyd,MDA)等氧化应激指标,实时定量PCR检测肝脏Ⅰ型胶原mRNA表达。结果与正常组相比,DMN模型组大鼠肝组织MDA含量及GST活性显著升高(P<0.01),分别为正常组的2.37倍和2.04倍,而GSH含量和SOD活性则显著降低(P<0.01),分别是正常组的0.37和0.47倍。与模型对照组相比,消黄方组显著降低肝组织MDA含量(P<0.05),增高GSH含量和SOD活性(P<0.01),实时定量PCR结果显示Ⅰ型胶原造模后达到正常组的34倍,消黄方组显著降低(P<0.01)。结论氧化应激是肝纤维化形成的重要病理因素之一,消黄方能显著改善DMN纤维化大鼠肝组织氧化应激。
Objective To investigate effects of Xiaohuang decoction in anti-oxidative stress of DMN-induced liver fi- brosis. Methods Male Wistar rats （n = 30） were randomly divided into normal group （10） and DMN-treated （20） group. At the third week, DMN-treated rats were randomly divided into DMN-water rats and DMN-xiaohuang decoction rats （10 rats each group）. At the end of the fourth week, all rats were sacrificed. The activity of superoxide dismutase （ SOD）, glutathione-S-transfcrase （ GST）, the contents of malondialdehyde （MDA） and glutathione （GSH） were deter- mined. The liver Hyp content was observed by Jamal＇ s methods. Results Compared with normal group, GST activity and MDA content increased significantly in DMN-water rats, while activity of SOD and GSH content decreased notably. Compared with DMN-water rats, Xiaohuang decoction improved these parameters significantly. Conclusion Oxidative stress is an important factor in liver fibrosis. Xiaohuang decoction can improve the anti-oxidative stress of DMN-induced liver fibrosis.
出处
《胃肠病学和肝病学杂志》
CAS
2012年第5期462-465,共4页
Chinese Journal of Gastroenterology and Hepatology
基金
国家十一五科技重大专项子课题(2009ZX10005-019)
上海市科学技术委员会项目(25382)资助
上海市科委引导类项目(10411963000)
上海市卫生局项目(20114Y020)
中央高校基本科研业务费专项资金资助(10F115)
关键词
氧化应激
消黄方
二甲基亚硝胺
肝纤维化化
Oxidative stress
Xiaohuang decoction
Dimethylnitrosamine
Liver fibrosis
