摘要
目的:探讨消黄方对急、慢性肝损伤所致黄疸的治疗作用。方法:以消黄方为研究对象,运用α-萘异硫氰酸酯(ANIT)诱导急性黄疸和二甲基亚硝胺大鼠肝硬化诱导慢性黄疸模型,观测消黄方的疗效。(1)急性黄疸:Wistar雄性大鼠40只,随机分为正常组(10只)和ANIT处理组(30只)。ANIT按照2mL/kg一次性经口灌胃,48h后ANIT处理组随机分为模型对照组,消黄方组及UDCA组(各10只),各组每天分别给予生理盐水、消黄方、UDCA治疗1周,实验结束处死全部大鼠。(2)慢性黄疸:Wistar雄性大鼠27只,随机分为正常组(10只)和DMN模型组(17只)。DMN模型组大鼠腹腔注射DMN(10mg.kg-1)共4周制备肝硬化伴随黄疸模型。造模2周末取正常和模型大鼠各3只作为药前观察,其余模型大鼠(14只)随机分为消黄方组和模型对照组(各7只);实验第4周末处死全部大鼠,获取样本,观察大鼠的死亡率及肝功能情况。结果(:1)急性黄疸模型结果发现,与正常组相比:ANIT模型组大鼠肝功能显著异常,ALT、AST、ALP、TBIL显著升高(P<0.05或P<0.01)。UDCA和消黄方显著降低血清TBIL含量(P<0.01),消黄方显著降低ALT、AST活性(P<0.05或P<0.01),UDCA改善不明显(P>0.05)。(2)血清TBIL含量及ALT、AST活性随DMN造模时间呈梯形增加(与正常大鼠比较,P<0.05或P<0.01);与4周模型对照组相比,消黄方显著降低血清TBIL含量及ALT、AST活性(P<0.05或P<0.01)。结论:具有清热利湿、活血化瘀治法的消黄方具有保肝降酶退黄作用,对急慢性肝损伤(黄疸)均有显著的疗效,为临床应用提供充分的实验依据。
Objective：To investigate the therapeutic effect of Xiaohuang Decoction（XHD）on the acute and chronic liver injury esp.jaundice in rats.Methods：The acute jaundice model was induced by ANIT and the chronic jaundice was induced by DMN.First,for acute jaundice,male Wistar rats（40）were randomly divided into normal group（10）and ANIT-treated group（30）.After 48h ANIT-treatment,ANIT-treated rats were randomly divided into model group,XHD group and UDCA group（10 in each）.At the end of the experiment,all rats were sacrificed.Second,for chronic janndice the rats were initially randomized into two groups： the control（n=10）and DMN-treated（n=17）groups.DMN was administered intraperitoneally to the DMN-treated rats for three consecutive days in each week.At the end of the second week,three rats from the control and DMN-treated groups were killed for the fibrosis development assessment.The remaining DMN rats were further randomized into two groups： the DMN-water group（n=7）,the DMN-XHD group（n=7）.Results：（1）for ANIT acute liver injury： compared with normal rats,ALT,AST,TBIL levels were significantly higher（P〈0.05 or P〈0.01）in ANIT rats.XHD and UDCA reduced remarkably TBIL content（P〈0.01）.XHD,but not UDCA decreased significantly ALT and AST activities（P〈0.05 or P〈0.01）.（2）For chronic DMN liver jaundice,compared with those of normal rats,ALT and AST activities and the content of TBIL in serum raised significantly,the content of TP and Alb in serum decreased remarkably in 4 weeks of DMN rats.Compared to 4 weeks of DMN rats,liver functions were improved significantly in XHD.Conclusion： Xiaohuang Decoction exerts significant inhibition on acute and chronic liver injury（esp.jaundice）in rats.
出处
《辽宁中医药大学学报》
CAS
2011年第11期50-52,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家十一五重大计划子课题(2009ZX10005-019)
上海市科学技术委员会资助项目(25382)
上海市科委引导类项目(10411963000)
中央高校基本科研业务费专项资金资助项目(10F115)
