摘要
为建立陕西卫矛高效再生体系,以幼茎为外植体诱导愈伤组织,在成功继代愈伤组织的基础上,进行愈伤组织再分化培养研究。采用正交设计方法,考察了6-BA、NAA及Ca2+3个因素对陕西卫矛愈伤组织再分化率的影响。结果表明:再分化培养基中添加3mg/L 6-BA+0.3mg/L NAA+5mmol/L Ca2+,能明显提高陕西卫矛愈伤组织的再分化率。
In order to establish a high efficient regeneration system,the stems of E.schensianus were taken as explants and induced callus formation after having plenty proliferated calli successfully,and then the calli were placed on different combinations regeneration medium.An orthogonal test was carried out to observe the influences of the 6-BA,NAA and Ca2＋ concentration on regeneration rate.The results showed that the suitable regeneration medium was MS containing 3.0 mg/L 6-BA,0.3 mg/L NAA and 5.0 mmol/L Ca2＋,it could obviously improve the frequency of regenerated shoots.
出处
《贵州农业科学》
CAS
北大核心
2012年第4期17-19,共3页
Guizhou Agricultural Sciences
基金
国家自然科学基金项目"斑马鱼母源性补体的传递和功能"(31000410)
陕西省教育厅2009年度科学研究计划项目"Ac/Ds转座元件转化猕猴桃的研究"(09JK434)
渭南师范学院大学生创新计划项目"几种观赏植物的组织培养"(11KX073)
关键词
陕西卫矛
组织培养
愈伤组织
再分化率
再生体系
Euonymus schensianus
tissue culture
callus
regeneration
regeneration
system
