摘要
目的:探讨HPVL1衣壳蛋白和P16在宫颈鳞状上皮内病变组织中的表达,及与疾病的诊断和预后的关系。方法:选取2010年4月～2011年9月我院妇科门诊就诊治疗的112例宫颈上皮内病变患者,其中CINⅠ级28例,CINⅡ级36例,CINⅢ级32例,宫颈鳞状细胞癌16例,采用免疫细胞化学法和免疫组化法分别检测患者病理组织学切片中HPVL1衣壳蛋白和P16的表达。并随访6～24个月。结果:各病理分级患者HPVL1衣壳蛋白、P16蛋白的阳性率差异均有统计学意义(P均<0.05),且HPVL1衣壳蛋白的阳性率与病理分级呈负相关,P16蛋白的阳性率与病理分级呈正相关。随访6～24个月,CINⅠ级HPVL1(+)P16(-)的患者疾病可自然消退,CINⅡ、Ⅲ级HPVL1(-)P16(+)的患者疾病具有进展为SCC的倾向,部分CINⅡ级HPVL1(-)P16(-)患者可进展为CINⅢ级,而HPVL1(+)P16(+)疾病均持续无进展。结论:HPVL1衣壳蛋白和P16可用于辅助诊断宫颈鳞状上皮内病变,联合检测这2个指标的阳性率可用于判断预后。
Objective： To investigate the relationship of between expression pattern of HPVL1 capsid protein and P16 in cervical squamous intraepithelial neoplasia and its the diagnosis and prognosis. Methods：A total ofl12 patients with cervical squamous intraepithelial neoplasia who were admitted to our hospital during April, 2010 to September, 2011 were selected in this research, of which 28 patients were CIN I , 36 and 32 patients were CIN II and CIN Ill respectively, and another 16 patients were cervical squamous cell carcinoma. Immunocytochemistry and immunohistochemistry were used to detect the expression of HPVL1 capsid protein and P16 in the pathological slice respectively, followed-up ranging from 6 to 24： months were conducted on all patients. Results： The positive rate of HPVL1 was negative related to pathological degree, while the positive rate of P16 was positive related to pathological degree. The following up showed the lesions of CIN I HPVLl（＋）P16（-）healed naturally; the lesions of CIN II and III progressed to squamous cell carcinoma; some CIN II HPVLl（-）P16（-） progressed to CIN III, while the other HPVL1（-）P16（-）patients and HPVL1（＋）P16（＋）had no progression. Conclusions. HPVL1 and P16 show diagnostic value in diagnosis, and when combined detected can predict the prognosis of cervical squamous intraepithelial neoplasia.
出处
《海南医学院学报》
CAS
2012年第5期607-609,共3页
Journal of Hainan Medical University
基金
深圳市宝安区科技计划项目(2010589)~~
