摘要
目的:建立乳香中α-蒎烯和乙酸辛酯含量测定方法,为制订乳香和制乳香质量标准提供参考。方法:采用气相色谱-质谱法测定,Rxi-5ms石英毛细管柱,进样口温度250℃,程序升温,起始温度50℃,保持1 min,以5℃.min-1程序升温至130℃,保持1 min。进样量1μL;分流比1∶60;载气为氦气。质谱电离方式为EI,离子源温度200℃,接口温度为250℃,电子能量70 eV,电离电压0.78 KV,扫描范围m/z 45～450。结果:以50倍量正己烷超声提取30 min;10批次乳香中α-蒎烯和乙酸辛酯的质量分数分别为0.021 3～0.149 5,2.519 6～9.098 0 mg·g-1,10批制乳香中分别为0.015 9～0.065 9和0.801 0～12.812 2 mg·g-1。结论:建立了乳香中α-蒎烯和乙酸辛酯含量测定方法,方法稳定、可靠。
Objective： To establish method for determining the contents of α-pinene and octyl acetate in Boswellia serrata, in order to provide preference for making quality standards for B. serrata and processed B. serrata. Method： Application of orthogonal design was employed to optimize the solvent, solvent quantity and extraction time. The GC-MS analysis was performed on a Rxi-Sms silica capillary column, running in the electron impact （El）mode, with ion trap and injector temperature of 200 ℃ and 250 ℃, respectively. The column oven was initially 50℃ and was held for 1 rain after injection, followed by temperature ramping at 5 ℃ · min^-1 up to 130 ℃, holding for 1 min. 1 μL of samples solution were injected in the split mode（ 1： 60）. Helium was the carrier gas. The mass spectrometer was set to scan m/z 45-450 with an ionizing voltage at 70 eV. Result： Sample solutions were prepared for 50-fold dose by ultrasonic extraction with hexane for 30 min. The content of a-pinene and octyl acetate in 10 batches of B. serrata were 0. 021 3- 0. 149 5, 2. 519 6-9. 098 0 mg · g^-1, respectively. And, those of α-pincne and octyl acetate in processed B. serrata were 0. 015 9- 0. 065 9, 0. 801 0-12. 812 2 mg · g^-1 Conclusion： The method is a stable and reliable for determining the contents of α-pinene and octyl acetate in B. serrata.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第10期1431-1433,共3页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2009ZX09103-314)
关键词
乳香
制乳香
Α-蒎烯
乙酸辛酯
含量
Boswellia serrata
processed Boswellia serrata
α-pinene
octyl acetate
content
