摘要
目的观察腹腔注射盐酸氨溴索对气管内注射阿米卡星后大鼠气管黏膜损伤的超微结构的影响。方法将30只Wistar大鼠经气管穿刺注入阿米卡星,随机分为对照组(阿米卡星组,15只)及氨溴索干预组(15只),经大鼠腹腔注射盐酸氨溴索注射液,在2、4、8、24及48 h 5个时间点处死大鼠各3只。取气管下1/3段的腹侧部,采用扫描电镜观察气道表面结构。结果阿米卡星组均出现不同程度的黏液分泌增加,黏稠度增加,纤毛倒伏、粘着在一起、排列紊乱、部分断裂缺失,恢复较慢,2、4、8、24及48 h受损面积百分比分别为98.2%、98.5%、97.5%、92.7%及82.1%。氨溴索干预组上述改变较轻,修复较快,2、4、8、24及48 h受损面积百分比分别为85.7%、81.9%、73.0%、61.9%及50.2%,恢复明显较对照组快。经气管注药在各时间点阿米卡星组纤毛受损面积明显高于氨溴索干预组(P<0.01)。结论阿米卡星可对大鼠气道黏膜表面结构造成严重损害,氨溴索可促进气管黏膜的修复,氨溴索注射液法治疗气管损伤效果理想,值得临床推广使用。
Objective To investigate the effects of ambroxol hydrochloride on surface structure of trachea mucosa in rats injured by intratracheally instilled amikacin.Methods Thirty Wistar rats injured by intratracheally instilled amikacin（0.252 mL / kg） were randomly divided into a control group（n = 15） and an ambroxol group（n = 15）.The rats in the ambroxol group were intraperitoneally injected with ambroxol hydrochloride（70 mg / kg） 5 minutes after amikacin administration.They were all equally divided into five subgroups and sacrificed at 2,4,8,28,48 hours respectively.Then the samples of 1 /3 lower segment of trachea were collected and observed under scanning electron microscope.Results In the control group,the mucous secretion and its stickness were increased.The cilia were found lodged,sticked together,aligned abnormally,abrupt partly,and recovered slowly,with the percentage of damaged area of 98.2%,98.5%,97.5%,92.7%,82.1% at 2,4,8,24,48 h,respectively.The injuries of mucosa in the ambroxol group were much milder and recovered more rapidly than those in the control group,with the percentage of damaged area of 85.7%,81.9%,73.0%,61.9%,50.2% at 2,4,8,24,48 h,respectively.Conclusions Intratracheal instillation of amikacin can cause cilia ultrastructure damage on tracheal mucosa.Ambroxol can promote the recovery process and alleviate airway inflammation.
出处
《中国呼吸与危重监护杂志》
CAS
2012年第3期274-277,共4页
Chinese Journal of Respiratory and Critical Care Medicine
基金
军队科技资金资助
