摘要
背景与目的:研究赛替派转化人支气管上皮细胞株(Humanbronchialepithelialcellstrain2B,BEAS-2B)过程中两种不同形态集落来源的细胞株的细胞构成和成瘤性的变化情况。材料与方法:赛替派转化BEAS-2B细胞过程中产生两种形态学上差异明显的集落,对其分离培养,细胞建株,分别命名为BEAS-S(HumanbronchialepithelialcellstrainS)和BEAS-C(HumanbronchialepithelialcellstrainC)细胞,连续传代,同时通过特异免疫细胞化学染色了解细胞构成的改变,结合细胞凝集性和锚着独立性的变化进行分析。结果:BEAS-C细胞随着传代,基底细胞标志物质蛋白34βE12表达增强,同时其细胞凝集性和成瘤性也增强;而BEAS-2B和BEAS-S细胞的细胞构成及成瘤性在传代过程中改变不明显。结论:基底细胞可能是人支气管上皮细胞恶性转化的干细胞,同时两种细胞集落形态的差异性也为人源细胞转化的形态学研究提供参考价值。
BACKGROUND&AIM: To study the changes of cellulosity and tumorigenicity of the two cell strains derived from different morphologic colonies in the process of malignant transformation of human bronchial epithelial cell strain2B(BEAS-2B)treated with thiotepa. MATERIAL AND METHODS: The two different morphologic colonies,which derived from transformed BEAS-2B cells by thiotepa,were isolated and cultivated respectively.We established the two cell strains and named them human bronchial epithelial cell strain S(BEAS-S)and human bronchial epithelial cell strain C(BEAS-C).With the passage,the immunocytochemistry stain,cellular agglutination test and anchorage independence test were used to analyze their changes of cellulosity and tumorigenicity. RESULTS: With the passage,the basal cell's special marker cytokeration34βE12express increased in BEAS-C cells as its cellular agglutination and tumorigenicity,but these don't change obviously in the BEAS-2B cells and BEAS-S cells. CONCLUSION: The basal cell might be the stem cell of malignant transformation of human bronchial epithelial cells.Meanwhile,the morphometric difference of these cells and colonies provided valuable information to the morphometrig research of transformation of human-originated cells.
出处
《癌变．畸变．突变》
CAS
CSCD
2005年第4期198-201,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金资助(No.30271559)
关键词
赛替派
永生化人支气管上皮细胞
恶性转化
形态计量学
免疫细胞化学染色
thiotepa
immortalized human bronchial epithelial cells
malignant transformation
morphometry
immunocytochemistry stain
