摘要
目的探讨家蝇幼虫分泌型抗菌肽对正常小鼠免疫功能影响。方法选用BalB/c小鼠,随机分为对照组(生理盐水)和家蝇幼虫分泌型抗菌肽(腹腔注射)5、10、20 mg/kg组,每天1次,7 d后处死小鼠,测量体重、胸腺/体重比值、小鼠腹腔巨噬细胞吞噬鸡红细胞能力、刀豆蛋白A诱导小鼠脾T淋巴细胞转化率、碳粒廓清能力及半数溶血值。结果 10、20 mg/kg抗菌肽组小鼠脾脏指数分别为(7.41±0.93)和(7.56±1.05),胸腺指数分别为(3.80±0.69)和(3.51±1.02),巨噬细胞吞噬率为(38.81±7.88)%和(40.16±9.74)%,脾脏酸性磷酸酶活性分别为(268.60±36.64)、(270.48±54.62)U/g,脾T淋巴细胞转化率分别为(0.31±0.11)%和(0.32±0.09)%,与对照组比较,差异有统计学意义(P均<0.05)。抗菌肽组小鼠体重及血清半数溶血值与对照组比较,差异无统计学意义(P>0.05)。结论家蝇幼虫分泌型抗菌肽对小鼠具有增强免疫功能作用。
Objective To study the influence of secreting type antibacterial peptides from Musca domestica larvae on the immune system of normal mice. Methods Thirty-two BALB/c mice were selected and divided randomly into four groups.Different doses of secreting type antibacterial peptides（5,10,20 mg/kg）were administered to the mice with intraperitoneal injection for 7 days.A series of classical tests were adopted to evaluate the effects. Results In the 10 mg/kg and 20mg/ kg dose groups,the ratio of spleen/body weight were 7.41 ±0.93 and 7.56 ±1.05;the phagocytotic ability of macrophages were 38.81±7.88 and 40.16±9.74;the lymphocyte transformation induced by conA of the mice were 0.31±0.11 and 0.32±0.09.All the indexes were higher than those of the control with statistically significant differences（P0.05 for all）.But the body weight and the 50 percent hemolyzingdose of complement of the 5-20mg/kg dose groups were not different from that of the control group. Conclusion The secreting type antibacterial peptides from Musca domestica larvae could promote the immune function in normal mice.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2012年第5期619-620,共2页
Chinese Journal of Public Health
基金
国家自然科学基金(30960343)
贵州省教育厅自然科学基金(F2008-6)
贵阳医学院基金项目(C2007-2)
贵州省科技厅基金(黔科合【2010】3160)
关键词
家蝇幼虫
分泌物
抗菌肽
小鼠
免疫功能
Musca domestica larvae
secretion
antibacterial peptide
mice
immune function
