摘要
设计合成了新的(2-(2'-吡啶)苯并咪唑)(L-丙氨酸根)铜(II)配合物:[Cu(HPB)(L-Ala)(ClO4)(H2O)]2 H2O[HPB=2-(2'-吡啶)苯并咪唑,L-Ala=L-丙氨酸根].应用元素分析、红外光谱、紫外可见光谱、摩尔电导率、电喷雾质谱及X射线单晶衍射等方法对配合物的组成及结构进行了表征.该配合物晶体属单斜晶系,P21空间群,晶胞参数:a=1.1900(2)nm,b=0.80500(16)nm,c=1.9700(4)nm,β=94.78(3)°,Z=2,Dc=1.672 g cm-3,F(000)=968,残差因子R1=0.0427,wR2=0.1106[I>2σ(I)],S=0.999.在配合物分子中,2-(2'-吡啶)苯并咪唑和L-丙氨酸根以双齿配位方式在分子平面上与中心铜(II)离子配位,而水分子及高氯酸根单齿弱配位于分子轴向上,构成了一拉长的八面体结构.利用二倍试管稀释法测定了配合物的抗菌活性,并且研究了配合物对pBR 322 DNA的断裂作用.结果表明,该配合物对枯草杆菌(B.subtilis,G+),金黄色葡萄球菌(S.aureus,G+),大肠杆菌(E.coil,G-)和沙门氏杆菌(Salmonella,G-)具有良好的抑制活性,最小抑菌浓度为50～80μg mL-1,在维生素C存在下能够通过羟基自由基OH氧化断裂pBR 322 DNA双螺旋结构.
A new copper（II） complex： [Cu（HPB）（L-Ala）（ClO4）（H2O）]2 H2O [HPB = 2-（2＇-pyridyl） benzimidazole,L-Ala=L-alaninate],was synthesized and characterized by elemental analysis,IR,UV-Vis,molar conductivity,ES-MS and single crystal X-ray diffraction.The crystal belongs to the monoclinic space group P21,with the crystal cell parameters： a=1.1900（2） nm,b=0.80500（16） nm,c=1.9700（4） nm,β= 94.78（3）°,Z=2,Dc=1.672 g cm-3,F（000）=968,residual factors R1=0.0427,wR2=0.1106 [I2σ（I）],S =0.999.The central Cu（II） ions for the complex show a distorted and elongated octahedral geometry in which two nitrogens of HPB and the carboxylate oxygen atom O and the amino nitrogen atom N of L-Ala bind at the molecular plane,a water molecule and one oxygen atom of perchlorate are coordinated at the axial site.The complex was tested for its ability to inhibit the growth of two gram-positive（B.subtilis,S.aureus） and two gram-negative（E.coil,Salmonella） bacteria by doubling dilutions method,and DNA interaction of the complex has been studied.The result showed that the MICs of the complex against the bacteria are in the range of 50～80 μg mL-1,and can cleave pBR 322 DNA in the presence of vitamin C with hydroxyl radicals as the active species.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2012年第8期973-979,共7页
Acta Chimica Sinica
基金
广东省科技计划项目(No.2009B020312010)
华南农业大学211工程重点项目(No.2009B 010100001)资助项目~~
