摘要
目的研究维持性血液透析患者输血传播性病毒（ＴＴＶ）感染状况，及进行其部分基因序列分析。方法设计 ＴＦＶＯＲＦ１部分基因引物，巢式 ＰＣＲ检测 ８０例血液透析患者及 １２例血液透析工作人员血清标本，将ＰＣＲ产物克隆、测序分析。结果血液透析患者ＴＴＶ感染率为３５％（２８／８０），１２名工作人员中有１例呈ＴＴＶ阳性（８．３％）。与日本株相比较，本实验分离株ＴＦＶ ＯＲＦ１部分基因同源性为９６．７％，ＴＴＶ合并ＨＢＶ感染者８例（８％），合并ＨＣＶ感染者５例（６％）；ＴＴＶ与ＨＢＶ、ＨＣＶ同时感染者２例（２％）．ＴＴＶ阳性患者中，有输血史者占５７％。结论血液透析患者中存在严重的ＴＴＶ感染，血液透析工作人员亦存在ＴＴＶ感染，ＴＴＶ可以与ＨＢＶ、ＨＣＶ重叠感染，ＴＴＶ感染者可能与输血有关，但ＴＴＶ的非血液传播同样应引起重视，
Objective To investigate the infection of transfusion transmitted virus(TTV) in maintenance hemodialysis patients and analyse a part of TTV DNA sequence. Method Denome DNA of TTV ORF1 was amplified in sera from 80 hemodialysis patients and 12 nurses of dialysis center using a nested polymerase chain reaction(PCR) assay with specific primers. After molecular cloning and sequencing of the PCR products, the genetic valuations of the TTV DNA were analyzed. Results 35 % (28/80)of hemodialysis patients and Eight. 3% (1/12) of dialysis operators were TTV DNA postitive. Compared with the reported Japanese isolate, the mucleotide sequence homologies of TFV isolates in this experiment was 96.7%. 8 patients had HBV and TTV infection, 5 patients HCV and TTV infection, while 2 patients had HBV, HCV and TTV infection. 57% of the TTV DNA positive patients had the transfusion history. Conclusions TTV infection is seriously in hemodialysis patients, of whom most have transfusion history, and can be found in dialysis operators. TTV can be co-infected with HBV and HCV. The infection of TTV without transfusion should be considered as well.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2000年第1期28-30,共3页
Chinese Journal of Nephrology
