摘要
以白皮松成熟胚的子叶作为外植体进行组织培养,利用秋水仙素诱导多倍体。将子叶预培养10 d后,用质量体积分数为0.01%、0.03%、0.05%的秋水仙素分别浸泡6 h、12 h、24 h,观察子叶生长状态及芽分化状况,20 d后鉴定染色体加倍情况。结果表明:不同浓度的秋水仙素均对子叶生长状态产生影响,浓度越高、处理时间越长,死亡率越高;秋水仙素处理过的子叶芽再生缓慢,且芽再生率随浓度增高而降低;秋水仙素处理后的子叶染色体产生加倍,但随着秋水仙素浓度的提高和处理时间的延长,多倍体诱导率呈现出下降的趋势。数据显示质量分数为0.03%的秋水仙素处理12 h的组合,多倍体诱导率最高,为40.5%。本研究初步建立了白皮松多倍体诱导体系。
Cotyledon explants of mature embryos of Pinus bungeana tissue were culture,the use of colchine induced polyploid.Cotyledons were cultured for 10 days,with the quality of volume fraction of 0.01%,0.03%,0.05% of colchicine immersion,respectively 6 h,12 h,24 h,observation of cotyledon growth and bud differentiation status,identification of chromosome doubling in 20 days.Results indicated that：different concentrations of colchicine effectfed cotyledon growth effect.Concertration was higher,processing time was longer,the higher the death;colchicine treated shoot regeneration is slow,and shoot regeneration rate increased with the concentration decreased;colchicine treated cotyledons chromosomes have doubled,but with colchicine concentration enhancement and treatment the extension of time,polyploid induction rate showed a downward trend.Data displayed quality score of 0.03% for the combination of 12 h treated with colchicine,polyploid induction rate was highest,40.5%.The study on polyploid induction of Pinus bungeana system were initially established.
出处
《山东农业大学学报（自然科学版）》
CSCD
北大核心
2012年第2期184-188,共5页
Journal of Shandong Agricultural University：Natural Science Edition
基金
山东省科技发展计划项目(2009GG10009009)
泰安市大学生科技创新行动计划项目(2010D2007)
关键词
白皮松
子叶
多倍体
秋水仙素
诱导率
Pinus bungeana
cotyledon
polyploid
colchicine
induction rate
