摘要
目的:探讨大蒜素对不同年龄组小鼠血清ACTH、CORT的影响。方法:健康ICR小鼠60只,随机分为青年组和老年组;各组又分设对照组和实验组。老年组鼠是用D-半乳糖制备衰老模型。实验组喂食大蒜素14 d,对照组灌服同体积生理盐水。采用ELISA法测各组小鼠血清ACTH、CORT含量。结果:各老年组鼠的血清ACTH、CORT水平均明显低于青年组鼠(P<0.05)。青年实验组鼠的ACTH、CORT水平较青年对照组明显升高(P<0.05),老年实验组鼠较老年对照组的ACTH、CORT水平数值上有升高,但差异无显著性(P>0.05)。结论:大蒜素对HPA轴有明显上调ACTH和CORT表达的作用,尤以青年组明显。
Objective：To observe the effects of garlicin on plasma adrerrmrticotropic hormone（ACTH） and corticosterone（CORT） of mice in different age groups.Methods：Sixty health ICR mice were randomized into aged or young group consisting of subgroups of treatment and controls.The aged group were administered with D-galactose for aging models and fed with oral garlicin for consecutive 14 days,whereas the control groups were managed with equal amount of normal saline.Enzyme-linked immunosorbent assay（ELISA） was used to detect the plasma concentrations of ACTH and CORT.Results：Both ACTH and CORT contents in the aged animals were found significantly lower than those in young ones（P0.05）.Elevated levels of ACTH and CORT were seen the in the treatment groups as compared with the controls（P0.05）,although the same results were seen in the aged mice treated by the agent compared to their counterparts,yet,the difference was minor（P0.05）.Conclusion：Garlicin can improve the release of ACTH and CORT through hypothalamo-pituitary-adrenal（HPA） axis in mice,and the influence appears significant in young animal.
出处
《皖南医学院学报》
CAS
2012年第2期89-91,共3页
Journal of Wannan Medical College
基金
安徽高校省级自然科学研究重点项目(kj2010A322)
