摘要
目的检测食管鳞状细胞癌组织及配对癌旁组织中c-Met蛋白的表达情况,探讨c-Met的表达与食管鳞状细胞癌患者临床病理特点的相关性。方法收集87对甲醛固定,石蜡包埋的食管鳞状细胞癌及配对的癌旁组织标本,所有病例均经病理证实,同时收集患者的临床资料。采用免疫组化的方法检测组织中c-Met的表达情况。结果 c-Met主要表达于细胞膜,表现为胞膜呈棕黄色染色。87例食管鳞癌组织中有29例(33.3%)呈阳性表达。配对癌旁组织中未见c-Met表达。c-Met的表达与食管鳞状细胞癌患者的肿瘤浸润深度、淋巴结转移及临床病理分期(tumor-node-metastasis,TNM)存在显著相关性(分别为P=0.017,P=0.000,P=0.000),而与患者的性别、年龄、肿瘤细胞分化程度、肿瘤大小等无明显相关性(P>0.05)。结论 c-Met在部分食管鳞癌组织中呈高表达,且与患者的肿瘤浸润深度、淋巴结转移及TNM分期存在显著相关性,c-Met的高表达可以作为高侵袭性食管鳞癌的分子标志物。
Objective To detect the expression of c-Met in esophageal squamous cell carcinoma tissues（ESCC） and paired non-cancerous tissues,and to investigate the association between c-Met and clinicopathological parameters of ESCC patients.Methods Eighty seven pairs of paraffin-embedded ESCC tissues and paired non-cancerous esophageal tissues were collected from the Department of Pathology,Beijing Friendship Hospital Affiliated To Capital Medical University.Sections from each specimen were examined by a pathologist and graded histologically.Patients＇ clinical parameters were collected meanwhile.Immunohistochemistry（IHC） was applied to detected the the expression of c-Met in the tissues.Results c-Met positive signals showed brown-yellow granules in the membrane.The expression of c-Met in non-cancerous esophageal tissues was always negative,whereas strong positive staining of c-Met was observed in 29/87（33.3%） of ESCCs.The expression of c-Met was correlated with tumor invasion depth,lymph node metastasis,and TNM（tumor-node-metastasis） stage（P=0.017,P=0.000,P=0.000,respectively）.No significant association between c-Met expression and other clinicopathological parameters（patients＇ gender,age,cell grading,and tumor size） was found（P0.05）.Conclusion The overexpression of c-Met was correlated with tumor invasion depth,lymph node metastasis,and TNM stage of ESCC patients.The overexpression of c-Met may be a marker for highly invasive ESCC.
出处
《首都医科大学学报》
CAS
2012年第2期223-226,共4页
Journal of Capital Medical University
基金
国家自然科学基金(81050016)
北京市科委资助项目(Z101100055610031)~~
