摘要
目的:建立变质甘蔗中3-硝基丙酸(3-NPA)的简便快速离子色谱法。方法:利用dionex1500型离子色谱仪,选用AS19阴离子分析柱,20 mmol KOH淋洗液,流速为1.00 ml/min,电导检测器。样品用纯水超声提取后,经高速离心0.2μm滤膜过滤后直接进样。结果:本法相关性好(r=0.9998),精密度高(RSD<3.0),样品平均加标回收率为96%～102%,定性检出限为0.03 mg/kg,定量检出限为0.08 mg/kg。结论:该方法简便快速、灵敏度高、选择性强,适用于变质甘蔗食物中毒分析。
Objective：To develop a simple and quick method for determination of the 3-Nitropropionic Acid in mildew sugarcane by ion chromatography.Methods：The ion chromatograph Dionex 1500 and AS19 analytical column and conductivity detector were applied.Using 20 mmol KOH as eluent,the flow rate was 1.00 ml/min.After ultrasonic extraction with pure water,the mildew sugarcane samples were centrifuged at a high speed,then filtered through a 0.2 μm membrane,finally injected directly.Results：This method exhibited excellent relativity（r=0.9998）and good analytic precision（RSD3.0）.The samples′ average recovery rates were in the range of 96%~102%.The detection limit（LOD）of the method was 0.03 mg/kg,and the limit of quantification（LOQ）was 0.08 mg/kg.Conclusion：This method was convenient,fast,sensitive and practical.It can be applied to the analysis for food poisoning of mildew sugarcane.
出处
《中国卫生检验杂志》
北大核心
2012年第4期711-712,共2页
Chinese Journal of Health Laboratory Technology
