摘要
目的 观察龈沟液 (gingivalcrevicularfluid ,GCF)中细胞外弹性蛋白酶 (elastaseinthesupernatant,EA s)和细胞内弹性蛋白酶 (elastaseinthepellet,EA p)在牙周基础治疗后的变化。方法用滤纸条的袋内取样法取 8例患者 43个牙位治疗前后的GCF样本 ,以底物检测法测定其中的EA s及EA p水平。结果 治疗后的GCF EA s、EA p总量及EA s浓度、EA s/EA p(S/P)比值均较治疗前显著下降 (P <0 0 0 1) ,但EA p浓度治疗前后无显著差异 (P >0 0 5 )。结论 GCF EA水平反映牙周组织的炎症破坏程度 ,可作为评价疗效的客观指标 ,尤其是EA s和S/P的作用明显 。
Objective To examine the changes of elastase in the supernatant (EA s) and in the pellet (EA p) after centrifuge in gingival crevicular fluid(GCF) before and after periodontal treatment Methods GCF was sampled with filter paper strips by intra pocket method to determine EA levels Forty three teeth of eight subjects were included EA s and EA p were determined seperately using the subtract Meosuc ala ala val pro NA Results EA s per site and per ml, EA p per site and the ratio of EA s/EA p(S/P) decreased significantly after non surgical periodontal treatment ( P < 0 001), while EA p concentration didn't change( P > 0 05) Conclusions GCF EA s and EA p could act as objective parameters to evaluate the effect of periodontal therapy, especially EA s and S/P, which may be sensitive diagnostic parameters for determining periodontal disease activity
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2000年第1期15-17,共3页
Chinese Journal of Stomatology
关键词
牙周炎
龋沟液
弹性蛋白酶
Periodontitis
Pancreatopeptidase
Gingival crevicular fluid
