摘要
目的 探讨白细胞介素 10 (IL 10 )对慢性淋巴细胞性甲状腺炎 (CLT)γ 干扰素 (IFN γ) /IL 4水平失衡的影响。方法 采用酶联免疫吸附法 ,对加入基因重组IL 10处理前后 10例CLT患儿外周血单个核细胞 (PBMC)诱生IFN γ和IL 4水平的变化进行观察。结果 患儿PBMC产生IFN γ为(30 81± 182 3)ng/L ,IL 4为 (2 89± 32 )ng/L ,IFN γ/IL 4比值为 10 .1± 5 .4;对照组IFN γ为 (15 0 6±5 78)ng/L ,IL 4为 (382± 10 4)ng/L ,IFN γ/IL 4比值为 5 .0± 3.1,两组比较差异有显著意义 (t =2 .6 1、3.5 6、2 .6 1,P <0 .0 5、0 .0 1、0 .0 5 )。加入终浓度为 2 0 μg/L的重组人白细胞介素 10 (rhIL 10 )后 ,IFN γ为 (1831± 15 6 2 )ng/L ,IL 4为 (381± 12 5 )ng/L ,与对照组比较差异无显著性意义 (P >0 .0 5 )。结论IL 10可抑制Th1类细胞因子IFN γ的产生 ,诱导Th2 类细胞因子IL 4的升高 。
Objective To explore the effect of IL 10 on the imbalance of IFN γ/IL 4 in children with chronic lymphocytic thyroiditis. Methods The levels of IFN γ, IL 4 in peripheral blood mononuclear cells (PBMC) before and after receiving recombinant interleukin 10 stimulation were determined by using ELISA in 10 children with chronic lymphocytic thyroiditis (CLT). Results The levels of IFN γ [(3 081±1 823)ng/L] and the ratio of IFN γ/IL 4 10.1±5.4 increased ( P <0.05) and IL 4 [(289± 32)ng/L] decreased ( P <0.01) in children with CLT as compared with controls [(1 506 ± 578)ng/L, 5.0±3.1, (382±104)ng/L]. The increased IFN γ and declined IL 4 were reversed by rhIL 10 (20 μg/L) to(1 831±1 562)ng/L and(381±125)ng/L , which were not significantly different from those of controls ( P >0.05). Conclusion IL 10 could redress the imbalance of Th 1/Th 2 type cytokine by inhibiting the synthesis of Th 1 type cytokine and inducing the production of Th 2 type cytokine.
出处
《中华儿科杂志》
CSCD
北大核心
2000年第2期97-99,共3页
Chinese Journal of Pediatrics
