摘要
本文提出了邻氨基酚 (OAP) -H2 O2 -辣根过氧化物酶 (HRP)伏安酶联免疫分析体系并用于烟草花叶病毒 (TMV)的测定。本方法以线性扫描二阶导数伏安法检测HRP催化H2 O2 氧化OAP的产物 ,用于HRP测定的检出限为 1 5× 1 0 - 1 mU·L- 1 ,线性范围为 2 5× 1 0 - 1 ～ 1 .0× 1 0 3mU·L- 1 。本法对TMV测定的检测限为 2 .0ng·mL- 1 。用所建立的方法测定病毒感染病叶澄清液的最高稀释比为 1∶3.9× 1 0
A voltammetric enzyme-linked immunoassay based on new system of o-aminophenol (OAP)-H 2O 2-horseradish peroxidase(HRP) has firstly been developed and used for the detection of HRP and tobacco mosaic virus(TMV).HRP or labelled HRP catalyzes the oxidation reaction of OAP with H 2O 2,the product of which produces a senstitve voltammetric peak at potential of -0.43 V(vs.SCE)in Britton-Robinson(BR) buffer solution.By using this voltammetric peak,HRP can be measured with a detection limit of 1.5×10 -1 mU·L -1 and a linear range of 2.5×10 -1 ～1.0×10 3 mU·L -1 .The detection limit for the clarified TMV is 2.0mg·L -1 and the highest dilution ratio detected for the infected leaf sap is 1∶3.9×10 6.
出处
《青岛化工学院学报（自然科学版）》
2000年第1期31-35,共5页
Journal of Qingdao Institute of Chemical Technology(Natural Science Edition)
基金
国家自然科学基金! ( 2 9775 0 12 )
山东省青年科学基金 !(Q99B16)
关键词
烟草花叶病毒
辣根过氧化物酶
伏安法
Tobacco Mosaic Virus(TMV)
Horseradish peroxidase(HRP)
O-aminophenol
Electrochemical Immunoassay
Voltammetry
