摘要
目的研究STC1基因对人宫颈癌CaSki细胞增殖及周期的影响。方法 CaSki细胞分为3组:实验组转染pcDNA3.1(+)-STC1质粒;空载体转染pcDNA3.1(+)空载体;空白对照组不加任何干扰。挑选稳定表达克隆,用RT-PCR及Western blot检测细胞中STC1基因表达水平;MTT法检测转染STC1基因对细胞增殖的影响;流式细胞仪检测转染STC1基因后细胞周期的变化。结果稳定转染后,与空载体组相比,实验组STC1基因的表达水平显著提高(P﹤0.01),CaSki细胞存活率下降(P﹤0.05),G1期细胞比例显著上升(P﹤0.05),细胞出现G1期阻滞。结论 STC1基因与CaSki细胞增殖相关,其高表达引起的宫颈癌细胞增殖速度降低可能与其所致的细胞G1期延长有关。
Objective To observe the influences of STC1 gene on the proliferation and cell cycle of human cervical cancer cell line CaSki. Method CaSki cells were divided into three groups： the experimental group transfected with plasmid pcDNA3.1（＋）-STC1;Empty vector group transfected with pcDNA3.1（＋）;blank control group transfected without any interference. The stable expression clones were chosen and the expression of STC1 gene was identified by RT-PCR and Western blot. The proliferation activities of the CaSki cells were assessed by MTT assay. Cell cycle was analyzed by flow cytometry. Results In comparison with the empty vector group, the expression of STC1 gene was significantly up-regulated in the experimental group after 48h transfection （P 0.01）. Over-expression of STC1 gene suppressed the growth of CaSki cells （P ﹤0.01）, and increased the G1-phnase cell proportion significantly （P 0.05）. CaSki cells were blocked at G1 phase. Conclusion STC1 gene is involved in the proliferation activity of CaSki cells. Over-expression of STC1 gene suppresses CaSki cell growth and the mechanisms may be due to the G1 phase arrest.
出处
《肿瘤药学》
CAS
2011年第2期125-128,共4页
Anti-Tumor Pharmacy
