摘要
本研究利用RNA干扰技术研究亚洲璃眼蜱p36基因在其生长、发育、繁殖方面的生物学功能。通过注射p36基因的dsRNA成功实现了该基因的表达沉默,干扰组表达量下降了99%。蜱p36基因沉默后,24 h的吸附率下降了50%,饱血率下降了55.5%,卵孵化率下降了100%。结果表明p36基因在亚洲璃眼蜱吸血和繁殖中具有重要作用。
In this study,the functions of p36 gene of Hyaloma asiaticum（Ha-p36） in growth,development and reproduction were studied using RNA interference（RNAi）.By the injection of double-strand RNA（dsRNA） of Ha-p36,gene silencing was confirmed by real-time PCR,which showed the expression amount of Ha-p36 gene decreased 99% after RNAi.Comparing with the control,the tick attachment rate,engorgement rate and egg hatching rate decreased 50%,55.5% and 100% in the RNAi group,respectively.The results indicated that Ha-p36 might play an important role in the growth and reproduction of H.asiaticum.
出处
《中国动物传染病学报》
CAS
2011年第3期49-53,共5页
Chinese Journal of Animal Infectious Diseases
基金
国家自然科学基金(30871846)
关键词
亚洲璃眼蜱
免疫抑制蛋白
RNA干扰
Hyalomma asiaticum
immunosuppressive protein
RNA interference（RNAi）
