摘要
根据GenBank中猪肺炎支原体(Mhp)J株P36蛋白基因(登录号X67286)的核苷酸序列设计1对引物,建立了快速检测Mhp的PCR方法。该方法能扩增出948bp的Mhp特异性条带,其敏感性达到可检测出0.735ng的Mhp DNA,但对鸡毒支原体、猪伪狂犬病毒、猪圆环病毒、猪流感病毒、猪呼吸与繁殖综合征病毒均未检测出相应目的条带;将克隆获得的目的片段与GenBank已发表的Mhp J株的P36基因进行比较,同源性达到99.9%。采用所建立的PCR方法对42份疑似猪支原体肺炎(MPS)病料进行临床诊断,发现有13份呈阳性(31.0%)。可见,该PCR方法适合于MPS的临床诊断,可为其防控提供可靠依据。
The present study was conducted to develop a PCR method for detecting Mycoplasma pneumoniae of swine （MPS） and its clinical applications. A pair of primer was designed for the detection of Mycoplasma hyopneumoniae （Mhp） according to the P36 protein gene sequence （Accession number： X67286） of J strain, and the PCR method for rapid detection of Mhp was developed. It was found that 948 bp of Mhp specific band can be amplified and 0.735 ng of Mhp DNA can be detected by the PCR assay, with no observation of the Myeoplasma galliseptieum, pseudorabies virus, eircovims, swine influenza virus, porcine reproductive and respiratory syndrome virus. The homology of cloned fragment was found to be 99.9% with the P36 gene of Mhp J strain （GenBank）. A total of 42 Mhp suspected pathogenic samples was collected from different places of Guangxi and diagnosed by the PCR assay. Out of 42 samples, 13 samples were positive for Mhp, which was accounting for 31%. Therefore, this PCR method could be applied for clinical diagnosis of the pathogen and provide basis for controlling MPS.
出处
《广西农业科学》
CAS
CSCD
2010年第3期256-258,共3页
Guangxi Agricultural Sciences
基金
广西科学研究与技术开发计划项目(桂科攻0537008-3B)
关键词
猪支原体肺炎
猪肺炎支原体
PCR
临床应用
Mycoplasma pneumoniae of swine
Mycoplasma hyopneumoniae
PCR
clinical application
