摘要
目的 观察碱性成纤维细胞生长因子 (basicFibroblastGrowthFactor,bFGF)对新霉素所致离体椭圆囊毛细胞损害的保护作用。方法 采用离体的豚鼠椭圆囊培养法 ,对照组为普通前庭培养液培养 ,实验组按培养液中含和不含bFGF分组 ,用新霉素造成各实验组培养的椭圆囊毛细胞损伤 ,各组椭圆囊均行扫描电镜检查并行毛细胞计数。结果 含bFGF的实验组毛细胞的存活数目显著高于不含bFGF的实验组 (P <0 .0 5 ) ,对照组毛细胞无缺失或损伤。结论 新霉素对离体的椭圆囊毛细胞有较强的破坏作用 ;
Objective To investigate the protective effect of basic Fibroblast Growth Factor(bFGF) on guinea pig utricular hair cells destroyed by Neomycin in vitro.Methods Neomycin was used to destroy guinea pig utricular hair cells in vitro.The culture in experimental groups was supplemented with bFGF.The culture in control group was not supplemented with bFGF.All utricles were observed by scanning electronic microscope,and the number of survival utricular hair cells was accounted.Results A significantly higher number of survival utricular hair cells was seen in experimental group(P<0.05),as compared with control group.Conclusion bFGF protected guinea pig utricular hair cells from Neomycin injury in vitro.
出处
《听力学及言语疾病杂志》
CAS
CSCD
2000年第2期71-73,共3页
Journal of Audiology and Speech Pathology
