摘要
本研究采用新疆细毛羊抑制素成熟区序列重组融合蛋白(roINH)作为免疫原,以Montanide(MON)和Freund’s(FA)作为佐剂,主动免疫SD大鼠,测定生殖激素及卵巢发育情况,评价佐剂效应。选用72只9～10周龄性成熟雌性SD大鼠,随机分为3组,分别皮下注射生理盐水(对照组)、roINH+MON(MON组)和roINH+FA(FA组)。各组大鼠每20d免疫1次,连续免疫3次。结果,MON组产生的抗体滴度显著高于FA组(P<0.05);与对照组相比,注射roINH+MON和roINH+FA均可极显著提高大鼠血清FSH平均含量(P<0.01),显著提高P峰值(P<0.05),而FSH及P平均含量及峰值在2个免疫组间无显著差异(P>0.05);LH含量和峰值在2个试验组间无显著差异(P>0.05);MON和FA组成熟卵泡数无显著差异(P>0.05),但均显著高于对照组(P<0.05);MON组脓包直径及炎症反应小于FA组。结果表明,应用roINH作为免疫原并配以MON或FA佐剂免疫大鼠均能取得较好的免疫效果,且MON佐剂炎症反应小于FA佐剂。
The aim of this study was to investigate the effect of active immunization using inhibin α subunit mature peptide（roINH） with Montanide（MON） and Freund＇s（FA） adjuvants on ovarian development in Xinjiang Fine-wool sheep.72 Sprague Dawley female rats of（9-10）-week-old were divided into 3 groups and actively immunized against the recombination fusion protein of Xinjiang Fine-wool sheep roINH with roINH＋MON adjuvant（MON group） or roINH＋FA adjuvant（FA group）,or injected with physiological saline（control group）,respectively.Each rat received a primary immunization and 2 booster immunization at 20-day interval.The results showed that,the antibody titer of MON group was significantly higher than that of FA group（P〈0.05）,compared to controls,mean concentration of FSH was higher（P〈0.01） in the MON and FA groups,and there was a significant increase in the peak of P in MON and FA groups（P〈0.05）,respectively.For the entire period of the study,mean concentration and peak of FSH and P were of no significant difference between the two immune groups（P〈0.05）;mean concentration and peak of LH between the two immune groups had no significant difference（P〈0.05）.There was no significant increase between MON and FA groups in the number of mature follicle（P〈0.05）,which were both significant higher than that in control group（P〈0.05）.Compared to FA group,the inflammatorial size was smaller and the inflammatory reaction was weaker in MON group.These results clearly demonstrate that active immunization against inhibin α subunit mature peptide in MON or FA adjuvants both can enhance immune effect,furthermore,the inflammatory reaction of MON adjuvant is less than that of FA adjuvant.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第4期558-563,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
四川省教育厅项目(09ZA072)
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848)
关键词
抑制素
主动免疫
佐剂
抗体
卵巢发育
inhibin
active immunization
adjuvants
antibody
ovarian development
