摘要
目的:运用反相高效液相色谱法(RP-HPLC)检测丹参五味子片中丹参酮ⅡA、五味子甲素的含量。方法:色谱柱选择Phenomenex KinetexC18(250mm×4.6mm,5μm),以甲醇-水为流动相,甲醇∶水为85∶15,流速设定为每分钟1ml。丹参酮ⅡA的检测波长为270nm,五味子甲素的检测波长为254nm;色谱柱温度设定为28℃;每次进样量为25μg;色谱柱量程为1.0AuFS。结果:丹参酮ⅡA和五味子甲素的线性范围分别为0.3232～1.616μg与0.525～2.625μg,平均回收率分别是99.08%、98.37%,RSD测定值分别为0.42%、0.73%。结论:运用反相高效液相色谱法可准确快速地对丹参五味子片中丹参酮ⅡA及五味子甲素的含量进行测定,操作简便,重复性好。
Objective：To set up a determination of tanshinoneⅡA and deoxyschizaadrin in danshenwuweizi tablet by RP-HPLC.Methods：Two components were separated through Kromasil C18（250 mm×4.6 mm,5 μm）column with methanol-water（85：15）by volume as a mobile phase.The flow rate was 1 ml/min and the detection wavelength were 270 nm（tanshinoneⅡA）and 254 nm（deoxyschizandrin）.Results：The linear range of tanshinoneⅡA and deoxyschizandrin were 0.323 2-1.616 μg and 0.525-2.625 μg,respectively.The average recovery was 99.08% with RSD 0.42% for tanshinoneⅡA and 98.37% with RSD 0.73% for deoxyschizandrin respectively.Conclusion：The method is simple,accurate and rapid with good reproducibility.It can be used for the quality control of danshenwuweizi tablet.
出处
《中外医学研究》
2012年第11期12-14,共3页
CHINESE AND FOREIGN MEDICAL RESEARCH
