摘要
利用RACE方法,从菊花(Chrysanthemum morifolium)‘神马’中分离得到细胞分裂素合成异戊烯基转移酶基因的全长cDNA序列,命名为DgIPT3,基因登录号为JQ711176。序列分析结果表明,DgIPT3的cDNA全长为1171bp,开放阅读框ORF编码331个氨基酸,具有IPT家族典型的ATP/GTP结合位点MGATGTGKS。系统进化分析显示,DgIPT3与毛果杨(Populus trichocarpa)的PtXP02321061亲缘关系最近。qRT-PCR分析表明,DgIPT3在菊花根、茎、叶中均有表达,其表达量为叶>茎>根。瞬时转化拟南芥(Arabidopsis thaliana)原生质体表明DgIPT3蛋白定位于细胞质中。过表达DgIPT3异源转化野生型拟南芥,莲座侧枝明显增多,表明DgIPT3可能是参与菊花侧枝形成的关键基因。
Branching is a limiting factor in highly effective production of Chrysanthemum. It is known that isopentenyl transferase is the key enzyme which catalyzes cytokinin biosythesis in plants. Here,we isolated full length cDNA of DgIPT3,a gene encoding isopentenyl transferase in Chrysanthemum using RACE. The DgIPT3 is 1 171 bp in length and its ORF encodes 331 amino acids. GenBank accession No. JQ711176. Phylogenetic analysis showed that DgIPT3 had the highest similarity with PtXP02321061 from Populus trichocarpa. qRT-PCR indicated that expression level of DgIPT3 in leaves is much higher than roots and stems,suggesting that expression pattern of DgIPT3 was organ-specific in Chrysanthemum. The DgIPT3 protein was located in cytoplasm by transient expression of DgIPT3 in mesophyll protoplast of Arabidopsis. Overexpression of DgIPT3 obviously increased the number of rosette branches in Arabidopsis,indicating that DgIPT3 is probably the key gene involved in branching of Chrysanthemum.
出处
《园艺学报》
CAS
CSCD
北大核心
2012年第4期721-728,共8页
Acta Horticulturae Sinica
基金
国家‘863’计划项目(2011AA100208)
教育部高等学校博士学科点专项科研基金资助课题(博导类)(20110008110021)
关键词
菊花
切花菊
异戊烯基转移酶基因IPT
亚细胞定位
组织特异性表达
过表达
Chrysanthemum
cut chrysanthemum
DgIPT3
isopentenyl transferases gene
subcellular localization
organ-specific pattern
overexpression
