摘要
目的探讨Notch信号传导途径配体基因JAG1和DLL1在结直肠癌的表达及其与临床病理特征和肿瘤预后之间的关系。方法回顾性分析2004年8月至2006年9月在南京中医药大学第三附属医院全国肛肠医疗中心行手术治疗的146例结直肠癌患者的临床及随访资料．建立患者肿瘤标本组织芯片并对JAG1和DLL1基因表达进行免疫组织化学检测。结果146例患者JAG1表达与其肿瘤分化程度有关．DLL1表达在不同肿瘤部位的表达差异有统计学意义（均P〈0．05）；两种基因表达与微卫星不稳定状态之间差异无统计学意义（P〉0．05）。134例（91．8％）患者获随访，随访时间（42．3±13．3）个月。无瘤生存86例（64．1％），1、3和5年总生存率分别为93％、74％和67％。多因素分析显示，患者预后与TNM分期、病理学类型、微卫星状态、JAG1基因表达有关（P〈0．05）。JAG1基因高表达患者预后优于阴性表达和弱阳性表达的患者（P〈0．05）。结论Notch信号转导途径配体基因JAG1和DLL1表达分别与肿瘤分化程度及肿瘤部位有关．JAG1基因与预后有关。
Objective To study the expression of JAG1 and DLL1 in colorectal cancer and its clinical significance. Methods Patients with colorectal cancer were treated in the Center of Colorectal Surgery of the Third Affiliated Hospital of Nanjing University of TCM were collected prospectively and followed up. A tissue microarray was made and expressions of JAG1 and DLL1 were detected by immunohistochemical staining. Results A total of 146 cases with colorectal cancer were included. The differences in JAG1 expression were significant among different tumor differentiation types and the differences in DLL1 expression were significant among different tumor locations （all P〈0.05 ）. There were no significant differences in the expression of the two genes and microsatellite instability（MSI）（P〉0.05 ）. One hundred and thirty-four （91.8%） cases were followed up and the mean follow-up time was （42.3±13.3） months. Tumor-free survival was noticed in 86 patients. The overall survival was 93% at 1 year, 74% in 3 years, and 67% in 5 years. Multivariate analysis showed that long-term survival rate was related to TMN stage, pathology types, MSI status and expression of JAG1. The prognosis of patients with high expression of JAG1 was better than those with low and negative expression （P〈0.05）. Conclusions The expressions of JAG1 and DLL1 are related to tumor differentiation and tumor location. The expression of JAG1 gene is associated with long-term survival.
出处
《中华胃肠外科杂志》
CAS
2012年第4期396-399,共4页
Chinese Journal of Gastrointestinal Surgery
基金
国家自然科学基金（30572447,30973837）
江苏省自然科学基金（BK2007009）
