摘要
目的探讨多重荧光PCR方法检测结直肠癌微卫星不稳定（MSI）及其临床意义。方法将2004-2005年间进行手术治疗的110例结直肠癌患者建立队列，以多重荧光PCR方法检测结直肠癌MSI，并对MSI和微卫星稳定（MSS）结直肠癌患者的临床病理特点进行比较。结果多重荧光PCR方法扩增出所有患者的5个微卫星序列。其中MSI．H10例（8．1％），MSI-L13例（11．8％），MSS为87例（79．1％）。共检测BAT．26变异9例（8．2％）、BAT．25变异11例（10．0％）、D2S123变异11例（10．0％）、D5S346变异6例（5．5％）和D17S250变异8例（7．3％）。MSI-L、MSI．H和MSS组结直肠癌患者年龄比较，差异有统计学意义（P〈0．05）；其他临床病理特点差异无统计学意义（P〉0．05）。结论多重荧光PCR方法检测MSI结果稳定，宜于临床应用；MSI和MSS结直肠癌患者临床病理特点比较未见差异。
Objective To detect microsatellite instability （MSI） in colorectal cancer by fluorescence multiplex polymerase chain reaction（FM-PCR） and explore its clinical value. Methods MSI of 110 colorectal cancer patients undergone surgical resection in our department from 2004 to 2005 were examined by FM-PCR, and the pathological characteristics were compared between MSI and microsatellite stabe（MSS） colorectal cancer patients. Results Among 110 cases, the male were 66 and the female were 44. Mean age was 60.8（26-94） yrs. AlP 5 microsatellite markers were amplified. Out of them, 10 cases（8.1%） were MSI-H, 13 cases（ll.8%） were MSI-L and 87 cases （79.1%） were MSS. Instability of BAT-26 was found in 9 cases （8.2%）, BAT-25 was in 11 cases（10.0%）, D2S123 was in 11 cases （10.0%）, D5S346 was in 6 cases （8.2%） and D17S250 was in 8 cases （7.3%）. Age between MSI and MSS colorectal cancer patients was significant and other pathological characteristics were not significant. Conclusions FM-PCR is a clinically stable method for MSI detection in colorectal cancer patients. There are no significant differences between MSI and MSS pathological characteristics of colorectal cancer patients.
出处
《中华胃肠外科杂志》
CAS
2007年第3期217-220,共4页
Chinese Journal of Gastrointestinal Surgery
基金
国家自然科学基金资助项目（30572447）
南京市医学发展重点项目资助课题（ZKX05028）
南京市科技人才培养项目（200407037）
关键词
结直肠肿瘤
聚合酶链反应
多重荧光
微卫星不稳定
Colorectal neoplasms
Polymerase chain reaction,fluorescence muhplex
Microsatellite instability
