摘要
目的检测乳腺浸润性导管癌(invasive ductal carcinoma,IDC)组织中尿激酶型纤维蛋白酶原激活剂(urokinase-type plasminogen activator,uPA)、基质金属蛋白酶-26(matrix metalloproteinase-26,MMP-26)的表达,计数微血管密度(microvessel density,MVD),探讨3项指标与IDC临床病理参数的关系,分析uPA、MMP-26表达的相关性及其与MVD的关系。方法采用免疫组织化学SP法检测85例IDC组织(观察组)及30例乳腺纤维腺瘤组织(对照组)中的uPA、MMP-26的表达,以CD105标记MVD。uPA、MMP-26蛋白表达与各临床指标的关系采用χ2检验,因MVD值为非正态分布,且方差不齐,两组及多组间样本均数比较采用非参数检验,蛋白表达相关性分析采用Spearman等级相关检验。结果 (1)uPA、MMP-26的阳性率分别为观察组62.4%(53/85)、64.7%(55/85),对照组26.7%(8/30)、16.7%(5/30),两组比较,差异有统计学意义(P均<0.05)。(2)IDC组织中uPA表达与IDC的淋巴结转移、组织学分级、pTNM分期有关(P均<0.05)。MMP-26表达与IDC的淋巴结转移、组织学分级、雌激素受体有关。uPA、MMP-26表达呈正相关(r=0.341,P=0.002)。(3)高MVD组uPA、MMP-26阳性率均明显高于低MVD组(P均<0.05)。结论 uPA、MMP-26在IDC组织中过表达,并且促进肿瘤血管生成。
Objective To detect the expression of urokinase-type plasminogen activator （uPA）, matrix metalloproteinase-26 （MMP-26）in breast invasive ductal carcinoma （IDC） tissue, measure the microvessel density （MVD） , investigate their correlation with clinical pathological parameters of IDC, the correlation of uPA and MMP-26 expression and the relationship with MVD. Methods Immunohistochemical SP method was applied to detect the expressions of uPA and MMP-26 in 85 cases of breast IDC （observation group） and 30 cases of breast fibroadenoma （ control group）. MVD was marked by CD105. Using SPSS17.0 statistical software, the relationship between clinical parameters and the protein expression of uPA, MMP-26 were analyzed by chi-square test. Because MVD had non-normal distribution and heterogeneity of variance, the sample means of different groups were analyzed by non-parametric test. Protein expression were analyzed using Spearman rank correlation test. Results （ 1 ） The positive rates of uPA and MMP-26 were 62. 4% （53/85） ,64. 7% （55/85） in observation group, and 26. 7% （8/30）, 16. 7% （5/30）in control group, respectively （all P〈0.05 ）. （2） In [DC tissues, the correlation was statistically significant between uPA expression and lymph node metastasis, histological grading, pTNM staging （all P〈0.05 ）. The correlation was statistically significant between MMP-26 expression and lymph node metastasis, histological grading, and estrogen receptor （ all P〈0. 05 ）. uPA expression had a positive correlation with MMP-26 expression （ r = 0. 341, P = 0. 002 ）. （ 3 ） The positive rates of uPA and MMP-26 in tissue with high MVD were significantly higher than those in tissue with low MVD （ P 〈 0. 05 ）. Conclusion uPA and MMP-26 are over-expressed in 1DC tissue, which can promote tumor angiogenesis.
出处
《中华乳腺病杂志（电子版）》
CAS
2012年第1期22-25,共4页
Chinese Journal of Breast Disease(Electronic Edition)
