摘要
目的:初步建立血浆循环DNA的提取及检测方法,探讨其在煤工尘肺发生发展过程中的作用。方法:选取煤工尘肺组(31例)、接尘工人组(38例)和正常对照组(40例)3组研究对象,采用磁珠法提取血浆循环DNA,实时定量PCR技术检测血浆循环DNA的相对水平,并根据Ct值绘出ROC曲线。结果:煤工尘肺组、接尘工人组和正常对照组血浆循环DNA的Ct值分别为18.00±1.07、19.20±0.58和19.80±1.57,煤工尘肺组血浆循环DNA的平均水平高于其他两组,差异具有统计学意义(P<0.05),根据煤工尘肺组与对照组数据所得ROC曲线下面积为0.839,煤工尘肺组与接尘工人组为0.859。结论:该方法能够方便快速的检测血浆循环DNA水平,血浆循环DNA水平的增高在煤工尘肺病的发生发展过程中具有重要的意义。
Objective： To establish a method of the extraction and detection of the plasma circulating DNA,and apply it to explore the role of circulating DNA played in the coal worker pneumoconiosis（CWP）. Methods： The cases were divided into coal worker pneumoconiosis group（31 cases）,dust workers group（38 cases） and the normal control group（40 cases）.Circulating DNA was extracted by the Immunoma-gnetic beads.Real-time polymerase chain reaction（Real-time PCR） technology was applied to detect the relative level of circulating DNA.According to the corresponding Ct value,the ROC curves were drawn. Results： The Ct values of coal worker pneumoconiosis group,dust workers group and normal group plasma were 18.00±1.07,19.20±0.58,19.80±1.57 respectively.There were significant differences among the three groups.The level of plasma circulating DNA in coal worker＇s pneumoconiosis group was higher than the other two.The area under the ROC curves drawn according to Ct value of the coal worker pneumoconiosis group and the normal control group was 0.839.The area of coal dust worker group and pneumoconiosis work-ers group was 0.859. Conclusion： The method could detect plasma DNA conveniently and rapidly.The increased levels of plasma circulating DNA was related to the occurrence and development process of CWP.
出处
《河南医学研究》
CAS
2012年第1期104-107,共4页
Henan Medical Research
基金
河南省科技攻关计划项目(2011020083)
