摘要
目的评价慢病毒转染的骨髓间充质干细胞诱导大鼠脊柱融合的能力。方法构建携带骨形态发生蛋白2（BMP-2）的慢病毒并将其转染到骨髓间充质干细胞中，观察细胞的碱性磷酸酶染色和活性。并将转染的细胞移植到大鼠脊柱融合模型中，8周后处死大鼠，对其脊柱标本进行手触力学测试，Micro-CT扫描和组织学切片观察。结果慢病毒转染细胞的碱性磷酸酶染色明显好于未转染细胞（3．2±0．4比1．1±0．2，P〈0．05）。转染的细胞移植到大鼠体内8周后，X线、Micro—CT、手触力学测试和组织学切片均显示转染的大鼠脊柱标本全部达到骨性融合。而未转染的大鼠脊柱标本均未融合。结论慢病毒转染的骨髓间充质干细胞能够诱导脊柱融合，可作为基因载体工具用于脊柱融合。
Objective To assess the spinal fusion in rats induced by bone marrow mesenchymal stem cells （BMSC） transfeeted with lentivirus. Methods Alkaline phosphatase （ALP） staining and ALP activity were determined in BMSC transfected with lentivirus encoding BMP-2 that were implanted to rat model of spinal fusion. At week 8, all rats were sacrificed. Thereafter, measurements of manual mechanics, Micro-CT scanning and histological analysis were performed. Results ALP staining appeared more satisfactory in BMSC transfeeted with lentivirus as compared with untransfected cells（3.2±0.4 vs 1.1±0.2, P〈0.05）. At week 8, spine bony fusion was achieved in all the transfected rats, as indicated by radiographic evaluation, Micro-CT and manual mechanics. In contrast, spinal fusion was absent in untransfected rats. Conclusion Lentivirus-transfected BMSC may achieve well-induced spinal fusion, and lentivirus vectors may offer the application of spinal fusion.
出处
《中华生物医学工程杂志》
CAS
2012年第1期16-19,共4页
Chinese Journal of Biomedical Engineering
基金
基金项目：江苏省卫生厅面上科研课题（H200916）
江苏省普通高校研究生科研创新计划资助项目（CX09B_038Z）
苏州市“科教兴卫”青年科技项目（SWKQ0905）
志谢感谢美国加州大学洛杉矶分校脊柱中心Jeffrey C Wang及Masashi Miyazaki两位教授对本合作课题的大力支持和帮助
关键词
慢病毒
骨髓间充质干细胞
脊柱融合
骨形态发生蛋白
Lentivirus
Bone marrow mesenchymal stem cell
Spine fusion
Bone morphogenetic protein
