摘要
目的:建立高效液相色谱法测定消乳癖颗粒中芍药苷的含量。方法:色谱柱:Kromasil C_(18)柱(250 mm×4.6 mm,5μm),流动相:乙腈-水(13:87),检测波长:230 nm,柱温为30℃,流速为1.0 ml·min^(-1)。结果:芍药苷在0.228～2.280μg范围内线性关系良好,r=0.999 9,平均回收率为99.9%,RSD=1.87%(n=6)。结论:该方法简便,结果准确,可用于消乳癖颗粒中芍药苷的含量测定。
Objective：To establish an HPLC method for the content determination of paeoniflor in Xiaorupi granules.Method：A Kromasil C_（18） column（250 mm×4.6 mm,5μm）was used.The mobile phase was acetonitrile-water（13：87）.The flow rate was 1.0 ml·min^（-1）.The detection wavelength was 230 nm and the column temperature was at 30℃Result：The linear range of paeoniflor was 0.228-2.280μg（r =0.999 9）.The average recovery was 99.9%with RSD of 1.87%（n=6）.Conclusion：The method is simple and accurate,and can be used for the content determination of Xiaorupi granules.
出处
《中国药师》
CAS
2012年第3期372-374,共3页
China Pharmacist
基金
国家重大新药创制项目(编号:2011ZX09401-305)
