摘要
目的:研究人参在生长季的不同时期茎中几种水解酶的活力变化。方法:以不同生长时期5年生人参茎为试验材料,采用中性磷酸缓冲溶液提取粗酶液,应用紫外分光光度法测定不同生长时期人参茎中淀粉酶(AMY)、酯酶(EST)、酸性磷酸酯酶(ACP)、碱性磷酸酯酶(ALP)和植酸酶(PHY)5种水解酶的活力。结果:人参茎中AMY活力在人参展叶期和开花期出现活力高峰,其他时期无明显变化;EST活力在人参展叶期、开花期和结果期出现活力高峰,果后参根生长期活力降低;ACP和ALP活力在人参生长季的每一时期均出现活力高峰;PHY活力在人参结果期出现活力高峰。结论:在人参不同生长时期茎中5种水解酶活力有其各自的变化特点,与人参的物质代谢、生长质量密切相关。
Objective：In order to research at changes of several hydrolase activity of ginseng stems in the different growth stages of growing season.Method： With five-year old ginseng stems as material,neuter phosphoric acid buffer solution was adopted to extract thick liquid enzymes.UV spectrophotometry was applied to determine the activities of amylase（AMY）,esterase（EST）,acid phosphatase（ACP）,alkaline phosphatase（ALP） and phytase（PHY） in ginseng stems in the different growth periods.Result： Activity peak of AMY was appeared in the spreading stage and the flowering stage,and no significant changes were found in other growth stage.Activity peak of EST was appeared in the spreading stage,the flowering stage,the fruit growth stage,and the activity was reduced in the root growing after fruit stage.Activity peak of ACP and ALP were appeared in every stage in the whole growing season.Activity of PHY was relatively high in the fruit growth stage.Conclusion： Five kinds of hydrolase activity of ginseng stems have their own characteristic changes in different periods,which may be closely related to material metabolism,growth quality of ginseng.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第7期135-138,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30873371)
关键词
人参
茎
水解酶
活力
Radix Ginseng
stem
hydrolase
activity
