摘要
为建立一套成本低廉、简单快速的分离检测土壤青枯病菌的方法,采用半选择性培养基(PCCG)和PCR技术相结合,从白肋烟栽培土壤中分离鉴定出12个菌株,并分析了这12个菌株的ITS序列。结果表明:分离出的12个菌株ITS序列与青枯病菌同源性最高,达到92.6%,该方法适合于土壤中青枯病菌的分离鉴定。Hayward的青枯病菌生化型鉴定结果表明,这12个菌株均为生化型Ⅲ。
In order to establish a method which is rapid and low in cost, for isolating and identifying the viable cells of Ralstonia solanacearum E. F. Smith （R. solanacearum） in hurley planted soil, a semiselective medium （PCCG）combined with polymerase chain reaction （PCR）method were used. 12 strains were acquired, purified, and identified by internal transcribed spacer （ITS） region and BLAST in NCBI. The results showed that the 12 strains were highly homologous to R. solanacearum （92.6%） , which suggested that the method was suitable for the isolation and identification of R. solanacearum in soil. Furthermore, the results of Hayward＇ s classification criterion of R. solanacearum showed that the 12 strains all belonged to biovar Ⅲ
出处
《烟草科技》
EI
CAS
北大核心
2012年第3期79-82,共4页
Tobacco Science & Technology
基金
四川省烟草专卖局资助项目"达州白肋烟主要根茎病害诊断及快速检测技术研究"(200901009)
关键词
白肋烟
土壤
青枯病菌
分离
鉴定
生化型
Burley
Soil
Ralstonia solanacearum
Isolation
Identification
Biovar
