摘要
[目的]研究母体孕期和哺乳期骨骼铅动员的规律及机制。[方法]36只雌性昆明小鼠通过饮水暴露于醋酸铅(含铅元素10.0mol/L)4周后怀孕,分别在孕期的第1、7和14天,哺乳期的第1、10和21天取股骨、全血和血清,用石墨炉原子吸收法测定铅含量,用放射免疫法测定总血清甲状旁腺激素(parathyroid hormone,PTH)含量,分别与同日龄非染铅母鼠比较。[结果]自孕中期开始,铅暴露组母鼠的血铅(blood lead)及血清总PTH水平开始明显上升,血钙、骨钙及骨铅水平开始明显下降;血铅水平在哺乳期末上升至(0.38±0.02)μmol/L,较孕初期上升65.22%(P<0.05);骨铅水平(femur lead)在哺乳期末下降至(269.14±9.78)nmol/g,较孕初期下降28.45%(P<0.05);血钙、骨钙水平在哺乳期末分别下降至(1.85±0.07)mmol/L、(12.74±0.38)mmol/g,较孕初期分别下降18.50%、17.75%(P<0.05);在孕中期,血清总PTH水平上升至(6.04±0.50)μg/L,较孕初期上升103.37%(P<0.05),直至哺乳期末仍维持在较高水平;两组的血铅、骨铅、血钙、骨钙及血清总PTH水平在观察期内各个阶段均有明显差异(P<0.05);铅暴露组血铅水平与血清总PTH水平之间有正相关关系(r=0.790,P<0.0001)。[结论]母鼠孕期及哺乳期骨骼铅动员可能与血清PTH水平的变化有关。
[Objective] To investigate the mechanism of bone lead mobilization in mice during gestation and lactation.[Methods] A group of 36 female Kunming mice were exposed to lead acetate in drinking water（10.0 mol/L,pure lead） for 4 weeks before pregnancy.At gestational day（GD） 1,2 and 14,and lactational day（LD） 1,10 and 21,levels of lead in blood and femur and levels of parathyroid hormone（PTH） in serum were measured by graphite furnace atomic absorption spectrometry and radioimmunoassay,respectively,and compared with the controls at the same time spots.[Results] In lead-treated mice,the levels of blood lead（PbB） and PTH were increased,and the levels of blood calcium（CaB）,femur calcium（CaF） and femur lead（PbF） were decreased during the second half of gestation.At the end of lactation,the levels of PbB were significantly increased to（0.38±0.02）μmol/L,up by 65.22%;the levels of PbF were significantly decreased to（269.14±9.78）nmol/g,down by 28.45%;the levels of CaB and CaF were significantly decreased to（1.85±0.07）mmol/L and（12.74±0.38）mmol/g,down by 18.50% and 17.75% respectively,compared with that on GD1（P 0.05）.The serum PTH were significantly increased to（6.04±0.50）μg/L on GD14,up by 103.37% compared with that on GD1（P 0.05） and maintained in a high level until LD 21.There were significant differences of PbB,PbF,CaB,CaF and PTH between the exposures and the controls in all stages of experiment（P 0.05）.Moreover,there was a positive correlation between the PbB and the serum PTH levels in dams with lead exposure（r=0.790,P 0.000 1）.[Conclusion] The elevated serum PTH levels might be involved in maternal bone lead mobilization during gestation and lactation.
出处
《环境与职业医学》
CAS
北大核心
2012年第3期139-143,共5页
Journal of Environmental and Occupational Medicine
基金
河北省计划生育委员会研究基金(编号:2011-A08)
河北省卫生厅科研基金项目(编号:20100037)
关键词
铅
骨
甲状旁腺激素
孕期
哺乳期
lead
bone
parathyroid hormone
gestation
lactation
