摘要
目的探讨转化生长因子β1(transforming growth factor-β1,TGF-β1)联合表皮生长因子(epithelial growth factor,EGF)诱导上皮向间质转化对人喉癌细胞株Hep-2侵袭能力的影响。方法应用TGF-β1单独刺激和TGF-β1联合EGF刺激人喉癌细胞株Hep-2后24、48 h观察细胞形态学的动态变化。应用Transwell检测细胞侵袭能力及RT-PCR和Western blot技术检测细胞上皮钙依赖黏附蛋白(E-cadherin)和波形蛋白(vimentin)的表达。结果①TGF-β1单独处理48 h组和TGF-β1联合EGF处理24、48 h组均能刺激喉癌细胞株Hep-2发生细胞形态改变。②TGF-β1联合EGF处理组无论是24 h还是48 h,其Hep-2细胞的侵袭能力都明显强于相同时段TGF-β1单独处理组(P<0.05);TGF-β1单独处理48 h组细胞侵袭能力又明显强于对照组(P<0.05)。③TGF-β1联合EGF处理组E-cadherin的表达降低,而Vimentin表达却明显上升。结论 EGF能够协同TGF-β1诱导上皮向间质转化,从而使喉癌具有更强的侵袭能力。
Abstract： Objective To investigate the effect of epithelialmesenchymal transition （EMT） induced by transforming growth factorβ11 （ TGF-131 ） cotreated with epithelial growth factor （ EGF ） on invasive ability of human laryngeal carcinoma cell Hep-2. Methods Human laryngeal carcinoma cell line Hep-2 was treated with TGF-β1 or TGF-β 1 ＋ EGF. Cellular morphological changes were observed seperatively under inverted microscope 24 h and 48 h after treatment. RTPCR and Western blot assay were used for estimating several invasive genes： E-cadherin and vimentin. The invasive ability was detected by transwell chamber. Results① EMT in Hep-2 was promoted both in the TGF-β1 group （48 h） and the TGF-β1 ＋ EGF group （24 and 48 h）. ② The enhancement of Hep-2 invasive ability in TGF-β1 ＋ EGF group （24 h and 48 h） were more obvious than that in TGF-β1 group（ P 〈0.05）. The invasive ability of TGF- β11group （ 48 h ） was more obvious than that of the control group （ P 〈 0.05 ）. ③Ecaclherin expression decreased and Vimentin expression increased obviously in TGFβ1 1 ＋ EGF group. Conclusion EGFcan promote EMT while co-treated with TGF-β11 , and therefore enhance the invasive ability of human laryngeal carcinoma cell line Hep-2.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2012年第1期10-14,共5页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
关键词
喉癌
细胞株
转化生长因子Β1
表皮生长因子
上皮向间质转化
Laryngeal neoplasm
Cell strain
Transforming growth factorβ1 1 （ TGF-j31 ）
Epithelial growth factor （ EGF ）
Epithelial- mesenchymal transition （ EMT ）
