摘要
目的:探讨细胞黏附分子CD44v6对膀胱癌T24细胞生物学行为的影响。方法:免疫细胞化学法检测正常膀胱移行上皮细胞和膀胱癌T24细胞中CD44v6蛋白的表达。用不同浓度的抗CD44v6单克隆抗体封闭细胞24、48和72h后,MTT法检测细胞的生长抑制率。FCM检测封闭前后细胞凋亡率,并通过免疫细胞化学法检测细胞凋亡相关蛋白Bcl-2和Bax表达水平的变化;Transwell小室检测细胞迁移特性的变化。结果:膀胱癌T24细胞表达CD44v6蛋白,而正常膀胱移行上皮细胞几乎不表达。MTT法检测结果显示,抗CD44v6单克隆抗体可抑制T24细胞的生长(P<0.01)。抗CD44v6单克隆抗体能增加T24细胞的凋亡率(P<0.01),并且Bcl-2蛋白表达降低,Bax蛋白表达增加(P<0.01)。抗CD44v6单克隆抗体封闭后T24细胞的迁移能力受到抑制,差异有统计学意义(P<0.05)。结论:抗CD44v6单克隆抗体封闭可以抑制膀胱癌细胞的增殖及迁移能力,同时诱导膀胱癌细胞凋亡,其机制可能与Bcl-2和Bax基因表达水平变化有关。
Objective： To investigate the effect of cell adhesion molecule CD44v6 on biological behavior of bladder cancer cell line T24. Methods： The expressions of CD44v6 protein in bladder transitional epithelial cells and bladder cancer T24 assay was used to detect the growth inhibitory rate anti-CD44v6 monoclonal antibodies for 24, 48 and cells were detected by immunocytochemistry. MTT of T24 cells treated with different concentrations of 72 h, respectively. After T24 cells were treated with anti-CD44v6 monoclonal antibodies, the apoptosis was detected by flow cytometer, the expressions of apoptosis-related markers Bcl-2 and Bax were determined by immunohistochemistry, and the change of migration ability ofT24 cells was detected byTranswell migration test. Results： CD44v6 was expressed in T24 cells, but rarely expressed in bladder transitional epithelial cells. MTT assay demonstrated that anti-CD44v6 monoclonal antibodies had inhibitory effects on the proliferation of T24 cells （P〈0.01）. The apoptosis rate of T24 cells was increased with an induction of anti-CD44v6 monoclonal antibodies （P〈0.01）. The expression level of Bax protein was up-regulated, while the expression level of Bcl-2 protein was down-regulated in T24 cells （P〈0.01）. The migration of T24 cells was inhibited after treatment with anti-CD44v6 monoclonal antibodies （P〈0.05）. Conclusion： Anti- CD44v6 monoclonal antibodies can inhibit the abilities of proliferation and migration of T24 cells, and also induce apoptosis. The mechanism may be related with the expression regulation of Bcl-2 and Bax genes.
出处
《肿瘤》
CAS
CSCD
北大核心
2012年第3期177-181,共5页
Tumor
基金
天津市应用基础研究计划资助项目(编号:07JCZDJC03400)
关键词
膀胱肿瘤
抗原
CD44v6细胞增殖
细胞凋亡
肿瘤转移
基因表达调控
Urinary bladder neoplasms
Antigens, CD44v6
Cell proliferation
Apoptosis
Neoplasm metastasis
Gene expression regulation
