摘要
[目的]研究资丘木瓜总黄酮提取工艺条件。[方法]以AlCl3试剂测定资丘木瓜总黄酮,在单因素试验的基础上,以总黄酮含量为指标,考察料液比、乙醇浓度、水浴温度等因素对提取效果的影响,并采用正交试验优选出最佳提取条件。[结果]影响资丘木瓜总黄酮的3个因素主次顺序为温度>料液比>乙醇浓度,其中温度和料液比对资丘木瓜黄酮类化合物的提取率影响较明显;最佳提取工艺为浓度50%乙醇为提取剂,水浴温度80℃,料液比1∶8(g/ml),提取时间3 h,提取次数4次;在此条件下,资丘木瓜总黄酮的提取率为1.348%。[结论]该试验确定的最佳工艺稳定性好且简便易行。
[ Objective ] To optimize the extraction process for total flavonoids from FRUCTUS CHAENOMELIS. I Method ] Aluminium trichlo- ride reagent was used to detect the total flavonoids in FRUCTUS CHAENOMELIS. On the basis of the single factor test, effects of solid-liquid ratio, ethanol concentration, extraction time, extraction temperature and extraction times on the extraction rate were studied by taking the content of total flavonoids as the index. [ Result] The influence degree of three factors on the extraction rate of total flavononids was in the order of temperature 〉 solid-liquid ratio 〉 ethanol concentration. Among them, temperature and solid-liquid ratio had relatively significant impacts on the extraction rate of total flavononids from FRUCTUS CHAENOMELIS. The optimal extraction process was 50 % ethanol concentration, 80℃ extraction temperature, 1：8 （g/ml） solid-liquid ratio, 3 h extraction time, and 4 extraction times. Under this condition, the extraction rate of total flavononids reached 1. 348%. I Conclusionl This method was simple convenient, and accurate for the detection of total flavonoids.
出处
《安徽农业科学》
CAS
2012年第9期5145-5147,共3页
Journal of Anhui Agricultural Sciences
基金
宜昌市医疗卫生科技项目(NO.A06425)
三峡大学青年科学基金项目(No KJ2008A001)
