摘要
目的:对自发性高血压大鼠(SHR)行星状神经节阻滞(SGB),观察星状神经节阻滞对其血压的影响。方法:将32只10周龄雄性SHR随机分为4组:左侧星状神经节阻滞组(LS组)、右侧星状节阻滞组(RS组)、药物卡托普利组(D组)和手术对照组(C组),每组8只。用ALC-NIBP无创血压测量系统测定大鼠血压,时间点包括基础值(T0)、第2周(T1)、第4周(T2)、第6周(T3)、第8周(T4)和第10周(T5)。实验结束后用3%戊巴比妥钠腹腔注射(45 mg/kg)麻醉SHR,迅速取出大血管,放射免疫法测定其eNOS和ET-1的浓度。结果:与基础值相比,LS组SHR血压逐渐升高(P<0.05或P<0.01);RS组血压第2周升高较明显(P<0.05),而其它时点较平稳,在167 mmHg～170 mmHg之间,与基础值(156±14)mmHg比较无明显改变(P>0.05);D组血压第2周和第4周下降(P<0.05),其它时点与基础值(156±16)mmHg相比无明显变化(P>0.05);C组血压各时点与基础值(154±12)mmHg相比均逐渐升高(P<0.01)。与C组和LS组相比,RS组大血管组织中ET-1表达明显降低(P<0.05),eNOS明显增强(P<0.05)。结论:阻滞右侧星状神经节能够明显降低血压,降低血管ET-1及升高eNOS蛋白的表达。
Objective： To investigate the effects of stellate ganglion block（SGB） on blood pressure in spontaneously hypertensive rats（SHRs）. Methods： Thirty-two 10-week-old male spontaneously hypertensive rats（SHRs） were assigned randomly into four groups：left stellate ganglion block group（Group LS）,right stellate ganglion block group（Group RS）,captopril group（Group D） and control group（Group C）.Arterial systolic blood pressure（SBP） was measured,and lendothelin（ET-1） and endothelial nitric oxide synthase（eNOS） in blood vessels were detected by radioimmunoassay. Results： Compared with baseline value,the blood pressure of Group LS gradually increased significantly（P0.05 or P0.01）;however,the blood pressure of Group RS was stable（P0.05）and increased only at week 2（P0.05）.The blood pressure of Group D decreased significantly at week 2 and week 4,and it remained stable compared with baseline value（P0.05）.The blood pressure of Group C gradually increased at weeks 2-10,compared with baseline values（P0.01）.Compared with Group LS and Group C,the expression of eNOS in blood vessels of Group RS significantly increased（P0.05）,and ET-1 decreased（P0.05）. Conclusion： The right stellate ganglion block can significantly lower blood pressure,down-regulate ET-1 and up-regulate eNOS protein expression.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2012年第1期65-68,共4页
Journal of Zhejiang University(Medical Sciences)
基金
安徽省教育厅自然科学研究项目基金(KJ2009B041Z)
