摘要
目的:用大鼠Walker-256移植性肝癌模型研究ilexgenin A抗肿瘤作用以及对肿瘤血管内皮生长因子(VEGF)和微血管密度(MVD)的影响,以探讨ilexgenin A抗肝癌作用机制。方法:32只大鼠移植性肝癌模型随机分为4组:ilexgenin A高、低剂量(100,500 mg.kg-1.d-1)组,5-Fu(5 mg.kg-1,隔日1次)组,模型组。于造模成功后第8天ilexgenin A治疗组及5-Fu组ip给药,模型组分别ig生理盐水10 mL.kg-1给药,连续用药14 d后停药24 h,取血处死大鼠,取出瘤块称重,常规瘤体组织形态学观察,分离血清检测肝功能指标天冬氨酸转氨酶(AST),丙氨酸转氨酶(ALT),用免疫组化法检测瘤块中VEGF和MVD的表达情况;另设正常组饲养大鼠8只,不进行造模及给药,供实验结束时作空白对照用。结果:ilexgenin A给药组瘤重明显低于模型组(P<0.01);ilexgenin A给药组及5-Fu血管条数目密集程度较模型组比较明显减少,ilexgenin A对血清AST,ALT活性升高有明显的拮抗作用,AST的含量与模型组比较差异显著(P<0.01);实验造模各组VEGF及MVD的表达水平均明显高于正常组(P<0.01);两者的表达均以模型组最高。结论:ilexgenin A可显著抑制大鼠体内癌细胞生长,对移植性肝癌所造成的肝功能损伤具有明显的保护作用,其作用机制可能与下调VEGF和MVD的表达水平有关。
Objective： To study the effect of ilexgenin A on vascular endothelial growth factor （VEGF） and microvessel density （MVD） of tumor in Walker-256 rat model to explore the mechansim. Method： Thirty two Walker-256 rats were randomly divided into 4 groups, and treated with the high dose of ilexgenin A （HD group）, low dose of ilexgenin A, 5-Fu （positive group） , and normal saline （NS group） , respectively. After 8 days of modeling, corresponding drugs were administered by intraperitoneal injection for 14 days. Then, blood rats werr executed to collect blood and remove tumors which were weighted, the morphological observation was performed, aspartate transaminase （AST） and alanine transaminase （ALT） levels were assayed, and the expression of VEGF and MVD in tumor was tested by immunohistochemical method. Result： In treatment group , the weight of tumor obviously decreased than that in model. In treatment group and 5-Fu , the vascular article number intensive degree, compared with model group, was obviously reduced, and ilexgenin A inhibited the elevated level of AST and ALT, the inhibition on AST level was more obvious （P 〈0. 01 ） , the expression of VEGF and MVD levels was significantly decreased compared with model group （P 〈 0.01 ）. Conclusion： Ilexgenin A can significantly inhibit growth of tumor, the mechanism may be related to down-regulating the expression of VEGF and MVD.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第6期196-199,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江西省自然科学基金项目(2010GQY0010)
江西省教育厅科技计划项目(GJJ11206)
