摘要
通过比较两种极性差异较大的基质2,5-二羟基苯甲酸(DHB)和2',6'-二羟基苯乙酮(DHAP)按不同比例混合时,AngiotensinⅡ的基质辅助激光解析电离-傅立叶变换离子回旋共振质谱(MALDI-FT/ICRMS)谱图的不同,并结合基质-AngiotensinⅡ在不同结晶方式下的共结晶和基质晶体的扫描电镜照片,发现基质为10μmol/L DHB和15μmol/L DHAP以体积比4:1组成的混合物时,基质结晶为致密的层状结构,而以薄层法与AngiotensinⅡ生成的共结晶,AngiotensinⅡ在基质晶体上面形成分散的柱状小晶体,此时得到的MALDI-FT/ICRMS质谱图优于干滴法。
Because of the suppression of 2′,6′-dihydroxyacetophenone（DHAP） to the combination of peptide with alkali metal ion and the advanced ability of 2,5-dihydroxybenzoic acid（DHB） to ionize peptide in MALDI-MS,it is illustrated in this work that the best MALDI-FT/ICRMS is obtained when matrix is composed of 10 μmol/L DHB and 15 μmol/L DHAP.The scanning electron microscope pictures of matrix with different compositions show that the crystal of matrix composed of 8 μmol/L DHB and 3 μmol/L DHAP is in a dense stratiform state that is quite different from crystal of matrix with other proportion of DHB and DHAP.It is also demonstrated that thin-layer method is the best peptide preparation method with matrix composed of 8 μmol/L DHB and 3 μmol/L DHAP among dry-droplet and sandwich method.
出处
《分析化学》
SCIE
CAS
CSCD
北大核心
2012年第3期421-426,共6页
Chinese Journal of Analytical Chemistry
关键词
基质辅助激光解析电离
傅立叶变换离子回旋共振质谱
肽
薄层法
干滴法
Matrix assisted laser desorption-ionization
Fourier transform ion cyclotron resonance mass spectrometry
Peptide
Thinlayer method
Dry-droplet method
