摘要
采用二倍稀释法测定了86种中草药对嗜水气单胞菌Aeromonas hydrophila的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),并采用正交试验法研究了对嗜水气单胞菌具有较强抑制作用的中草药配伍。结果表明,五倍子、五味子和乌梅对嗜水气单胞菌的抑制作用最强,最小抑菌浓度和最小杀菌浓度均为12.5mg/ml,可作为防治水产养殖动物嗜水气单胞菌病害的首选中草药。正交试验结果表明,儿茶、五倍子、五味子和乌梅4种药分别以4∶2∶4∶1和1∶2∶2∶4的比例配伍时对嗜水气单胞菌具有最佳抑菌和杀菌作用。
ABSTRACT The minimal inhibitory concentration （MIC） and the minimal bactericidal con- centration （MBC） of 86 kinds of Chinese herbal medicines （CHMs） on Aeromonas hydrophila were examined in vitro by the microdilution method. Then the optimum mixtures of CHMs which can inhibit the A. hydrophila were found through orthogonal test design. The result showed that Galla chinensis, Fructus schisandrae and Fructus rnurne had the strongest inhibito- ry effect on A. hydrophila,with the MIC and MBC as 12.5 mg/ml. In conclusion, G. chinen- sis, F. schisandrae and F. murne were the better drugs that can cure the disease caused by the A. hydrophila in aquatic animals. The results of orthogonal tests showed that the mixture of Acacia catechu, G. chinensis, F. schisandrae and F. rnume with the proportion of 4 ：2 ： 4 ： 1 and 1 ： 2 ： 2： 4 had the best MICandMBConA. hydrophila.
出处
《渔业科学进展》
CSCD
北大核心
2012年第1期114-121,共8页
Progress in Fishery Sciences
基金
宁波市农业科技攻关项目(2010C10057
2010C91050)
宁波大学学科项目(XKL121
XKL11D2099)共同资助
关键词
水产养殖
中草药
嗜水气单胞菌
最小抑菌浓度
最小杀菌浓度
正交试验
Aquaculture
Chinese herbal medicine （CHM）
Aeromonas hydrophila
Minimal inhibitory concentration （MIC）
Minimal bactericidal concentra-
