摘要
目的比较三叶青松散型和致密型愈伤组织悬浮培养细胞生长及其次生代谢产物黄酮的积累情况。方法将三叶青叶诱导的愈伤组织在不同外源植物激素种类和配比的培养基上多次继代培养,筛选获得松散型和致密型愈伤组织,分别建立相应悬浮细胞体系,研究了两种悬浮培养细胞生长和总黄酮积累的动态。结果三叶青松散型愈伤组织悬浮培养的继代周期为8 d,增加质量倍数最高达到3.68倍,黄酮积累的最大值11.7 mg/g,较最初接种时的浓度增加34.5%,该悬浮系继代4～5次后生命力衰退且褐化加重;而致密型愈伤组织悬浮培养的继代周期为20 d,增加质量倍数最高达到2.65倍,黄酮积累的最大值40.9 mg/g,较最初接种时的浓度增加212.2%,该悬浮系继代多次细胞无明显变化。结论与松散型愈伤组织悬浮培养相比,致密型愈伤组织悬浮培养更有利于继代培养和次生代谢产物的积累。
Objective To provide a basis for improving the quality of Alisma orientale in Sichuan Province by studying its quality and rhizosphere soil physicochemical property and analyzing their relevance correlation.Methods Determination of alisol B-23-acetate by RP-HPLC and water,ethanol extracts,total ash,and acid insoluble ash in A.orientale in Sichuan Province was carried out according to Chinese Pharmacopoeia 2010.And to investigate the physicochemical property of rhizosphere soil in growing area of A.orientale by the conventional methods.At last,to analyze the data by SPSS 17.0.Results All the contents of water,alisol B-23-acetate,total ash,and acid insoluble ash in A.orientale in Sichuan Province could meet the standard of Chinese Pharmacopoeia 2010,except the ethanol extracts.The total ash was significantly negative correlation to ethanol extracts,while significantly positive correlation to acid insoluble ash,the relative coefficients were 0.407 and 0.466,respectively.The contents of available K were short in all soil of growing areas for planting A.orientale.There was extremely significantly positive correlation between soil organic matter and alkali-hydrolysable N,total P,and available P,while significantly positive correlation between total K and available K,the relative coefficients were 0.555,0.806,and 0.433,respectively.Every single factor of all soil physicochemical property was affected by other soil factors highly.The total K of soil had the highest influence on the content of each component in A.orientale in Sichuan Province and the following was total N.Conclusion The quality of A.orientale in Sichuan Province in every place is good and steady,which could provide the reference for making the quality standard for A.orientale in Sichuan Province.The total K is influenced by the available K,but has the significant influence on A.orientale in Sichuan Province.However,all of the fields where planted A.orientale in Sichuan Province are short of available K.Therefore,to ensure the quality of A.orientale in Sic
出处
《中草药》
CAS
CSCD
北大核心
2012年第3期577-580,共4页
Chinese Traditional and Herbal Drugs
基金
浙江省新苗人才计划项目(ZJPCKJCX201006)
浙江医药高等专科学校校级科研项目(ZPCSR2010003)
关键词
三叶青
松散型
致密型
愈伤组织
悬浮培养
黄酮
Tetrastigma hemsleyanum Diels et Gilg
loose
compact
callus
suspension culture
flavonoid
