摘要
目的探讨血管内皮生长因子（VEGF）和水通道蛋白1（AQP1）在类风湿关节炎（RA）滑膜组织中的表达及其与滑膜血管生成的关系。方法采用免疫组织化学技术检测7例RA，12例骨关节炎患者关节滑膜组织中VEGF和AQP1的表达，对VEGF，AQP1阳性细胞数及血管数的表达进行评分，并比较它们之间的相关性。统计学方法采用秩和检验及Spearman相关分析。结果RA滑膜VEGF阳性细胞数与血管数呈正相关（rs=0．971，P〈0．01），骨关节炎滑膜VEGF阳性细胞数与血管数无相关性（L=0．235，P=0．462），VEGF阳性细胞数在RA与骨关节炎间差异无统计学意义（Z=0．390，P=0．711）；RA滑膜AQP1阳性细胞数与血管数呈正相关（rs=0．828，P=0．022），骨关节炎滑膜AQP1阳性细胞数与血管数无相关性（rs=0．396，P=0．203），AQP1阳性细胞数在RA与骨关节炎间差异无统计学意义（Z=-0．302，P=0．773）；RA中，VEGF阳性细胞数与AQP1阳性细胞数呈正相关（n=0．891，P=0．007），骨关节炎中，VEGF阳性细胞数与AQP1阳性细胞数无相关性（n=0．338，P=0．283）。结论VEGF，AQP1与RA滑膜血管的生成密切相关，二者在RA病情的发生和发展过程中有重要作用。另外在RA中，VEGF和AQPl还可能有协同作用。
Objective To study the expression of vascular endothelial growth factor （VEGF） and aquaporin 1 （AQP1） in the synovium of rheumatoid arthritis （RA） patients, and their correlation with angiogenesis. Methods The expression of VEGF and AQP1 in the synovial tissue was detected with i mmunohistochemistry in 7 patients with RA, 12 patients with osteoarthritis （OA）. The expression of VEGF and AQP1 positive cells and vessels was scored, and the correlation of positive cells with vessels was analyzed. Rank-sum test and Spearman＇s correlation analysis were used for statistical analysis. Results The expression of VEGF positive cells was significantly correlated with vessels in the synovium of RA group （rs=0.971, P〈 0.01 ）. No significant correlation was found between VEGF positive cells and vessels in synovium of the OA group （rs=0.235, P=0.462）. There was no evident difference in the expression of VEGF positive cells between RA and OA group （Z=-0.390, P=0.711）. The expressions of AQP1 positive cells were significantly correlated with vessels in the synovium of RA group （rs=0.828, P=0.022）. No significant correlation was found between AQP1 positive cells and vessels in the synovium of OA group （rs=0.396, P=0.203）. There was no notable difference in the expression of AQP1 positive cells between RA and OA group （Z=-0.302, P=0.773）. The expressions of VEGF positive cells were significantly correlated with AQP1 positive cells in RA group （rs= 0.891, P=0.007）. No significant correlation was found between VEGF positive cells and AQP1 positive ceils in OA group （rs=0.338, P=0.283）. Conclusion The expressions of VEGF and AQP1 are significantly correlated with angiogenesis in the synovium of RA, and they play an important role in the pathology of RA. Moreover, there may be synergistic effect between VEGF and AQP1 in RA.
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2012年第3期200-202,F0003,共4页
Chinese Journal of Rheumatology
关键词
血管内皮生长因子A
水孔蛋白类
关节炎
类风湿
新生血管化
病理性
Vascular endothelial growth factor A
Aquaporins
Arthritis, rheumatoid
Neovas- cularization, pathologic
