摘要
目的研究按摩对兔股四头肌损伤的肌组织病理修复、细胞骨架蛋白结蛋白(Desmin)和α-肌动蛋白(α-Actin)表达的效应差异,探讨按摩促进肌肉损伤修复的作用机制。方法健康成年雄性新西兰大白兔27只,体重(2.0±0.5)kg,随机分为3组,正常对照组(A组,n=3)、自然恢复组(B组,n=12)和按摩组(C组,n=12)。A组实验动物不作任何处理,B、C组实验动物用自制打击器制备兔右后肢股四头肌损伤模型。C组于造模后第8天开始按摩,按摩转速3 000～3 100 r/min,按摩时间15 min,每天1次至处死取材为止;B组不予按摩。B、C组于伤后14 d及21 d各取6只实验动物股四头肌样本,HE染色观察组织病理变化,免疫组织化学染色及Western blot检测Desmin、α-Actin表达,并与A组正常股四头肌标本进行比较。结果 B、C组实验动物均存活至实验完成。A组肌纤维形态规整,排列整齐,无结缔样组织;B组股四头肌标本见明显组织坏死,多数肌纤维断裂、萎缩、排列紊乱;C组骨骼肌组织形态、肌萎缩较B组改善,受损部位可见新生肌纤维。免疫组织化学染色显示各时间点B、C组Desmin、α-Actin表达均低于A组(P<0.05);但C组Desmin、α-Actin表达均较B组增强(P<0.05),且21 d时明显高于14 d(P<0.05)。Western blot检测示A组Desmin、α-Actin高于B、C组(P<0.05),B组两种蛋白表达最低。结论按摩可促进兔损伤股四头肌的组织形态及细胞骨架恢复,可能通过上调Desmin、α-Actin表达促进骨骼肌组织损伤修复。
Objective To investigate the effect of massage on quadriceps femoris repair and the expressions of Desmin and α-Actin in rabbits so as to explore the possible molecular mechanisms of massage in repair of muscle injury.Methods Twenty-seven New Zealand white rabbits,weighing（2.0 ± 0.5） kg,were randomly divided into 3 groups： groups A（n=3）,B（n=12）,and C（n=12）.In group A,the rabbits were not treated as controls;in groups B and C,the rabbit models of quadriceps femoris injury were prepared by self-made beater.In group B,no massage therapy was given as nature recovery controls;in group C,RT-N2 intelligent massage device was used for massage therapy at 8 days after injury,at 3 000-3 100 r/min for 15 minutes,every day for 7 days or for 14 days.The quadriceps femoris specimens were taken from 6 rabbits of groups B and C at 14 days and 21 days,respectively.HE staining was employed to detect the histomorphological change.Immunohistochemistry staining and Western blot were used to detect Desmin and α-Actin expressions.The massage therapy effect was evaluated by the histomorphological change and Desmin and α-Actin expressions.Results All rabbits survived to the end of experiment in groups B and C.No histological change was found with regular order of muscle fibers and no connective tissue in group A;obvious tissue necrosis was seen with broken muscular fibers,muscle atrophy,and irregular order in group B;and in group C,the skeletal muscle morphology and muscle atrophy were obviously improved with regenerated muscle fibers when compared with group B.Immunohistochemistry staining showed that the Desmin and α-Actin expressions obviously reduced in groups B and C,which were significantly weaker than that in group A（P 0.05）;the Desmin and α-Actin expressions were significantly stronger in group C than in group B（P 0.05）,and at 21 days than at 14 days in group C（P 0.05）.Western blot results showed that the Desmin and α-Actin expressions were significantly higher in group A than in groups B and C
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2012年第3期346-351,共6页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金资助项目(30940096)
重庆市教育委员会科学技术研究项目(KJ100307)~~
关键词
按摩
骨骼肌损伤修复
细胞骨架蛋白
兔
Massage Skeletal muscle injury repair Cytoskeletal protein Rabbit
