摘要
目的:探讨Ghrelin在小鼠妊娠围植入期子宫表达的变化及其对胚胎着床的影响。方法:用免疫组化方法检测Ghrelin蛋白在小鼠妊娠围植入期子宫表达的变化;采用体外胚泡培养方法,观察重组Ghrelin蛋白对胚泡的孵化、黏附与扩展率的影响。结果:在小鼠妊娠的第1至2天,Ghrelin蛋白主要于腔上皮和腺上皮表达,随妊娠进展,其表达水平逐渐下降。体外胚泡培养48h时,与正常对照组相比[孵化率、黏附率和扩展率分别为(61.25±5.27)%、(71.45±6.56)%和(43.26±5.87)%],Ghrelin重组蛋白可明显抑制胚泡的孵化率[(20.36±4.78%),P<0.01]、黏附率[(53.23±4.98)%,P<0.01)]及扩展率[(18.34±7.12)%,P<0.05]。结论:Ghrelin可抑制胚泡孵化、黏附与扩展及抑制胚泡植入。
Objective：To investigate the expression of Ghrelin in peri-implantation mouse uteri and the effect of Ghrelin on the implantation of mouse blastocysts in vitro. Method： Ghrelin distribution was detected by immunohistochemistry in peri-implantation mouse uteri. The effect of recombinant Ghrelin on blastocyst hatching, attachment and outgrowth was meas- ured by using blastocysts in vitro culture system. Result：Ghrelin was mainly located in luminal epithelium and glandular epithelium of mouse uterus in D1-D2 of pregnancy. With the progress of pregnancy, its level gradually decreased. Compared with the control group, recombinant Ghre- lin protein could significandy inhibit blastocyst hatching（61.25±5.27% ,20.36±4.78%, P〈 0.01 ）, attachment （ 71.45±6.56%, 53.23±4.98%, P 〈 0.01 ） and outgrowth （43.26±5.87%, 18.34±7.12% ,P〈0.05 ）. Conclusion： Ghrelin may participate in mouse embryonic implantation by inhibiting blastocyst hatching, attachment and outgrowth.
出处
《现代妇产科进展》
CSCD
2012年第2期124-127,共4页
Progress in Obstetrics and Gynecology
基金
江西省科技支撑计划基金项目(No:2009BSB10910)
